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Method for evaluating tobacco seed vigor through chemical inhibition

A technology for tobacco seeds and seeds, applied in the fields of seed and rhizome treatment, germination equipment, application, etc., can solve the problems of high precision requirements of test instruments, inability to be suitable for batch testing of enterprises, and poor control of repeatability, so as to improve the resistance of seeds. Inverse performance, reducing the risk of substandard seedling emergence, and improving seed quality

Active Publication Date: 2021-01-01
YUXI ZHONGYAN TOBACCO SEED
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  • Application Information

AI Technical Summary

Problems solved by technology

The seed vigor detection methods recommended by ISTA, such as accelerated aging, direct conductivity measurement, etc., are not suitable for the detection of tobacco seed vigor, and adversity stress methods such as compound stress method, control deterioration method, etc. are more effective in measuring tobacco seed vigor. Good, but there are problems such as poor control of repeatability and high requirements on the accuracy of test instruments, which cannot be applied to batch testing in enterprises

Method used

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  • Method for evaluating tobacco seed vigor through chemical inhibition
  • Method for evaluating tobacco seed vigor through chemical inhibition
  • Method for evaluating tobacco seed vigor through chemical inhibition

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0029] Experimental Example 1 Vitality Evaluation Test of Tobacco Seeds

[0030] 1. Experimental materials: Tobacco seeds were selected from the improved tobacco variety Yunyan 85, and the improved tobacco variety A was from Yuxi China Tobacco Seed Co., Ltd.

[0031] 2. Experimental method:

[0032] 1) Three batches of improved tobacco variety A produced in 2001, 2003, and 2008 were taken, respectively, for standard germination test, floating seedling cultivation test, and chemical reagent treatment. Each batch had 100 tobacco seeds, and each group of experiments was repeated 3 times for each batch.

[0033] Standard germination test: place two layers of filter paper in a petri dish, moisten it, place 100 tobacco seeds evenly on the filter paper, repeat 3 times for each batch number, then place the petri dish in an incubator at 25°C, light for 12 hours a day, during the germination period Keep the filter paper moist. The number of seeds germinated was recorded, and the radic...

experiment example 2

[0051] Experimental Example 2 Comparison of Vitality Evaluation Results and Emergence Rates of Different Varieties of Tobacco Seeds

[0052] 1. Experimental materials: Tobacco seeds were selected from tobacco varieties Yunyan 85, Yunyan 87, K326, and Yunyan 105. The improved tobacco varieties Yunyan 85, Yunyan 87, K326, and Yunyan 105 were all from Yuxi China Tobacco Seed Co., Ltd.

[0053] 2. Experimental method:

[0054] The standard germination test, the floating seedling test, and the chemical inhibition method vitality detection were carried out with reference to Example 1. The chemical inhibition method vitality detection only used the optimal treatment conditions, 0.01 mM 28-brassinolide soaked for 12 hours.

[0055] Data analysis: SPSS17.0 software was used to analyze the significance of the difference between the standard germination results of different batches of seeds, the emergence rate of floating seedlings, and the test results of chemical inhibition seed vigor;...

Embodiment 1

[0060] Select 3 batches of Yunyan 85 produced in 2001, 2003, and 2008, each with 100 tobacco seeds, put the above-mentioned 3 batches of seeds into beakers, add 0.01mM 28-brassinolide aqueous solution, and submerge Seeds were stirred, left standing, and soaked for 12 h; then the soaked tobacco seeds were poured into a nylon mesh bag, the residual liquid on the surface was rinsed with clean water, put in an oven at 30°C, blasted for 1 h to 2 h, and air-dried until The water on the surface of the seeds disappears and the seeds can disperse freely without forming agglomerates; use a seeder to spread the air-dried seeds evenly in a petri dish, lay two layers of wet filter paper in the petri dish, keep the filter paper moist during germination, and place it at 25°C for cultivation In the box, the light intensity of 1000 lx was irradiated for 12 hours every day, and it was kept in the dark for 12 hours; the vigor value of different batches of seeds of the fine variety Yunyan 85 could...

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Abstract

The invention discloses a method for evaluating tobacco seed vigor through chemical inhibition. The method specifically comprises the following steps of 1) seed pretreatment, namely soaking tobacco seeds with a 28-brassinolide aqueous solution, and then drying; 2) seed germination, namely putting the dried tobacco seeds into an incubator for conventional germination culture for 6-8 days; and 3) seed vigor determination, namely counting the number of normal germinated seeds after germination culture is completed, and determining the seed vigor value according to the following formula. The method has the beneficial effects that a regression coefficient value of a chemical inhibition method activity detection value calculated through the method and the emergence rate is the highest, is significantly related, can better represent the performance of seeds after encountering stress in the actual seedling raising process, and can be used as a standard to evaluate the seed quality, greatly improve the seed quality, improve the seed stress resistance, and reduce the risk that the emergence rate does not reach the standard after the seeds encounter stress in the tobacco seedling raising process. The method for evaluating the tobacco seed vigor has the characteristics of low cost, quickness, easiness in operation, accuracy and high efficiency.

Description

technical field [0001] The invention belongs to the technical field of tobacco seed quality detection, in particular to a chemical inhibition evaluation [0002] Methods of Tobacco Seed Vitality. Background technique [0003] Seed vigor refers to the comprehensive characteristics of different aspects of seed batches with high germination rate that can be measured when they emerge in a wide range of environments. Seed vigor is related to the rate and uniformity of seed germination, the ability to emerge under adverse conditions, and the ability to germinate after storage. Vitality is a further requirement and improvement of seed quality on the basis of high germination rate. At present, although tobacco is mostly planted in intensive sheds for floating seedlings, seeds may still encounter adversities such as low temperature and excessive water during seedling cultivation. Even seeds with a high germination rate will still have poor emergence after encountering adversity. To...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/00
CPCA01C1/00A01C1/025
Inventor 潘威杨晓东宋碧清杜景诚乔雨宋薇郑昀晔马文广
Owner YUXI ZHONGYAN TOBACCO SEED
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