Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Epitope peptide of tropomyosin antigen, and application of epitope peptide

A tropomyosin and epitope peptide technology, which is applied to the epitope peptide of tropomyosin antigen and its application field, can solve the problem that mollusk allergens are less than crustacean allergens, etc., achieve good application prospects, reduce pathogenic Sensitivity effect

Pending Publication Date: 2020-12-29
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Crustaceous aquatic food is one of the eight common food allergens, so research on seafood allergens is mainly focused on crustacean allergens, but molluscs in seafood and aquatic products can also cause allergic reactions
Crustaceans and molluscs have cross-allergens, according to research reports, but research on mollusk allergens is much less than crustacean allergens

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Epitope peptide of tropomyosin antigen, and application of epitope peptide
  • Epitope peptide of tropomyosin antigen, and application of epitope peptide
  • Epitope peptide of tropomyosin antigen, and application of epitope peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Detection of whether there is an antibody to the tropomyosin antigen of the present invention in the sample

[0033] 1. Materials and Methods

[0034] Serum samples from 10 subjects with oyster allergy were obtained from Plasma Lab International, USA. All 10 subjects had a clinical history of at least two or more symptoms within 1 h of ingesting oysters, including pruritus, allergic rhinitis, gastrointestinal disorders, and diarrhea. Venous blood from 10 patients with oyster allergy was collected and pooled to prepare a serum pool. Serum samples (from Plasma Laboratories International, USA) from 5 normal volunteers without any history of shellfish allergy were used as negative controls. Each serum sample was stored in aliquots at -80°C until use.

[0035] Experimental serum pool: Serum samples from 10 patients with oyster allergy were mixed in equal volumes.

[0036] Control serum pool: 5 serum samples without any history of shellfish allergy were mixed in...

Embodiment 2

[0058] Construction of the modified tropomyosin antigen of embodiment 2

[0059] All 5 IgE-binding epitopes were deleted to construct 5 peptide-deleted modified tropomyosin antigens, which were named as CrD224. The mutant only contained 224 amino acid residues, specifically, it was identified by SEQ ID No.14 Amino acid sequence shown:

[0060] SEQ ID No. 14:

[0061] MDSIKKKMIAMKMEKENAQDRAEQLEQQLRDTEEQKAKIEEDLHSNLENEFDTVNEKYQECQQSLNRRIQLLEEDATEKLEEASKAADESERNRKVLENLEKQLTEAKLIAEEADKKYDEAARKLAITEVDLERAEARLEAAEAKVYELEEQLSVVANNIKTLQIRDLTQRLKDAENRATEAERTVSKELAGERQKEVDR

[0062] The specific experimental process for constructing modified tropomyosin antigens with five peptide deletion mutations is as follows:

[0063] The entire protein sequence of Cra g 1 contains 284 amino acids, and the above-mentioned 5 specific IgE binding sequences (peptides 11, 9, 3, 6, 7) are deleted in the entire protein sequence, and the specific amino acid sequence is located in Cra g 1 44-49 , Crag 1 ...

Embodiment 3

[0064] Comparison of the reactivity of antigen Cra g 1 and its mutant CrD224 (i.e. modified tropomyosin antigen) with serum IgE in embodiment 3

[0065] 1. Experimental materials: the blood of 10 oyster allergic subjects, and the serum sources of 5 normal volunteers without any history of shellfish allergy are the same as in Example 1.

[0066] Experimental serum pool: equal volumes of serum from 10 subjects with oyster allergy were mixed and prepared.

[0067] Control serum pool: 5 serums without any history of shellfish allergy were prepared by mixing equal volumes.

[0068] Mouse sera: 10 mouse sera were obtained from Plasma Lab International.

[0069] 2. Synthesis of Epitope Peptides

[0070] The sequence and synthesis of the five peptides of SEQ ID No.1-5 and each reference peptide are the same as in Example 1.

[0071] 3. Comparison of the reactivity of antigen Cra g 1 and its mutant CrD224 with serum IgE

[0072] ① Antigen coating: First, dilute the antigen (Crag 1 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides epitope peptide of a tropomyosin antigen, and an application of the epitope peptide. The epitope peptide of the tropomyosin antigen comprises: (a) an amino acid sequence selected from SEQ ID No. 1-5; or (b) an amino acid sequence having at least 80% identity with the amino acid sequence selected from SEQ ID No. 1-5, can be used for developing tropomyosin with reduced sensitization, or used for detecting whether an antibody of the tropomyosin antigen exists in a sample, or used for detecting whether a subject is allergic to the tropomyosin antigen, and has good application prospects.

Description

technical field [0001] The present invention relates to an epitope peptide and its application, in particular to an epitope peptide of tropomyosin antigen and its application. Background technique [0002] Food allergy is an allergic reaction after people ingest specific food ingredients, which can cause clinical symptoms in sensitized patients, such as urticaria, asthma, abdominal pain and diarrhea, shock and even life-threatening. Nowadays, with the development of society, people's dietary structure has changed, and the incidence of allergic diseases caused by food is increasing, which poses a huge threat to people's health. Among them, the proportion of people allergic to aquatic products is quite large. Crustaceous aquatic food is one of the eight common food allergens, so the research on seafood allergens mainly focuses on crustacean allergens, but molluscs in seafood and aquatic products can also cause allergic reactions. According to research reports, crustaceans an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435G01N33/68A61P37/08
CPCC07K14/43504G01N33/6854A61P37/08G01N2333/43504
Inventor 谷瑞增李国明方磊马勇刘文颖陈亮刘艳
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com