Method for detecting transgenic components of corn by utilizing quadruple real-time fluorescent PCR
A technology of transgenic ingredients and real-time fluorescence, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems that cannot meet the needs of genetically modified food supervision
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[0063] 1 Materials and methods
[0064] 1.1 Sample collection
[0065] The maize positive samples were mainly "MON810", "MON863", "NK603", "MON88017", "Bt176", "Bt11", "FAPAS GeMSU 56A", "FAPAS GeMSU 56B", "ACAS-T067-J273". The maize negative samples were "ZY1711", "Meiyu No. 7", "Cainuo No. 1", "Jingnian No. 1" and "Tiannuo No. 182". The corn deep-processing products are the circulating products purchased by the laboratory in the market in 2019, with a total of 50 batches.
[0066] 1.2 Genomic DNA extraction
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