A method for extracting sweet tea antioxidant polyphenols

An extraction method and a technology of oxidizing polyphenols, which are applied in anti-toxic agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of long production cycle, large amount of ethanol consumption, large consumption of organic solvents, etc., and achieve energy consumption And the effect of high cost, long production cycle and shortened extraction time

Active Publication Date: 2021-10-08
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the Chinese patent with the publication number CN101190276 introduces a method of obtaining an extract from dry sweet tea by alcohol extraction and water precipitation. This method has the disadvantages of long production cycle, large amount of ethanol, complex process, high cost, and low product purity; The Chinese patent whose publication number is CN108752231A introduces a method for extracting polyphenols from sweet tea by adsorption method. The disadvantages of this method are: high cost and low purity; The method of fresh leaf polyphenols, the disadvantage of this method is: the number of extractions is high, the temperature is high, and it is easy to cause the loss of polyphenols
In summary, a few existing related extraction methods have the following defects: long time, large consumption of organic solvents, high cost, low yield, low safety, and low purity
Therefore, the current extraction process of sweet tea antioxidant polyphenols needs further research and improvement

Method used

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  • A method for extracting sweet tea antioxidant polyphenols
  • A method for extracting sweet tea antioxidant polyphenols
  • A method for extracting sweet tea antioxidant polyphenols

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Extraction of sweet tea antioxidant polyphenols of the present invention (small test)

[0033] Take sweet tea, dry it at 50°C, grind it at 14500rpm for 2min, pass through a 100-mesh sieve to get tea powder, seal it in a dry container, and store it at 4°C for later use; put 1g of sample powder into a centrifuge tube and mixed with 35mL ethanol aqueous solution (60%), after microwave extraction at 50°C for 25min, the mixture was cooled with running water, centrifuged at 4500xg for 8min, and the supernatant was collected to obtain the extract. The microwave power was 400-900W . The extract was diluted 5 times, and the antioxidant activity of the diluted sample was measured by TEAC method and FRAP method, and then the total phenol content of sweet tea was determined by the improved Folin phenol method. The values ​​of TEAC, FRAP and TPC were 613.113±9.321 μmol Trolox / g, respectively. 381.290±4.419μmol Fe 2+ / g, 135.940±0.516mg GAE / g.

Embodiment 2

[0038] Example 2 Extraction of sweet tea antioxidant polyphenols of the present invention (pilot test)

[0039] Take an appropriate amount of sweet tea, dry it at 50°C, grind it at a speed of 14500rpm for 2min, pass through a 100-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C for later use; mix 200g of sample powder with 8L of ethanol The aqueous solution (60%) was mixed, and after microwave extraction at 50°C for 25 minutes, the mixture was cooled with running water, centrifuged for 8 minutes, and the supernatant was collected to obtain the extract. The microwave power was 400-900W. The extract was diluted 5 times, and the antioxidant activity of the diluted sample was measured by TEAC method and FRAP method, and then the total phenol content of sweet tea was measured by the improved Folin phenol method. ±5.014mmol Fe 2+ / g, 51.8421±0.906g GAE / g.

[0040] The beneficial effects of the present invention will be described below through test e...

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Abstract

The invention discloses a method for extracting sweet tea antioxidant polyphenols, which comprises the following steps: 1) taking sweet tea, drying, crushing and sieving to obtain tea powder; 2) taking step 1) tea powder, adding ethanol aqueous solution and microwave extraction , centrifuged, and the supernatant was collected. The sweet tea obtained by the preparation method of the present invention has high content of antioxidant polyphenols and strong antioxidant activity. It has been proved by experiments that the sweet tea antioxidant polyphenols of the present invention are better than TEAC, FRAP and TPC without microwave assistance (soaking method) to extract tea polyphenols. The values ​​are 13.35%, 35.30% and 50.05% higher, respectively.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to an extraction method of sweet tea antioxidant polyphenols. Background technique [0002] Lithocarpus litseifolius (Hance) Chun, also known as sweet tea, sweet leaf tree, sweet tea, many spikes Lithocarpus Lithocarpus litseifolius (Hance) Chun, belongs to the genus Lithocarpus litseifolius (Hance) Chun. In my country, it is mainly distributed in Guangxi, Yunnan, Sichuan and Hunan. The main components of Mujiangye Ketian tea are polyphenols (8.9%), flavonoids (3.2%), rubusoside (1.5%), etc. It is a good health tea, and its antioxidant polyphenols have high development value. According to reports, Mujiang Yeke has good effects in anti-oxidation, antibacterial, hypoglycemic, hypolipidemic, anti-allergic, anti-viral, improving blood circulation and memory, and is safe and non-toxic. In recent years, the scope of application of Mujiang Yeke has been expanding, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P39/06
CPCA61K36/185A61K2236/333A61K2236/51A61P39/06
Inventor 甘人友刘毅夏宇刘宏艳
Owner INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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