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A method for aseptic germination and rapid propagation of Michelia majestic seeds

A magnificent seed technology, applied in the field of aseptic germination and rapid propagation of Michelia grandis, can solve the problems of narrow distribution area, scarce population, insufficient seedlings, etc., and achieve easy seedling formation, simplified reproduction steps, and effective reproduction rate high effect

Active Publication Date: 2021-05-14
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for aseptic germination and rapid propagation of Michelia grandis seeds, which can solve the problems of narrow distribution area of ​​Michelia grandis in the wild, scarce populations, and insufficient seedlings

Method used

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  • A method for aseptic germination and rapid propagation of Michelia majestic seeds
  • A method for aseptic germination and rapid propagation of Michelia majestic seeds
  • A method for aseptic germination and rapid propagation of Michelia majestic seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A method for aseptic germination of magnificent miraculous seeds, comprising the following steps:

[0050] Select the mature unsplit aggregate fruit of Magnificent Miracle, disinfect the surface with 75% alcohol on the ultra-clean bench, place it on sterile filter paper, dry the moisture on the surface, and take out the red fake fruit with sterile tweezers and blades. The seeds of the seed coat are explants.

[0051] Inoculate the seeds with red arils on the germination medium for germination culture, the medium is MS + no hormone; 1 / 2MS + no hormone; 1 / 3MS + no hormone; 1 / 4MS + no hormone; pure agar + no hormone, The pH values ​​were all 5.8. One seed was inoculated per bottle, and a total of 10 seeds were inoculated for each treatment, repeated 3 times.

[0052] Table 1 The screening of the germination medium of Michelia grandis

[0053]

[0054] Cultivate under the conditions of no light and a temperature of 23-30°C. After 10 days, the seeds begin to germinate....

Embodiment 2

[0056] A method for rapid propagation of magnificent miraculous seeds, comprising the following steps:

[0057] The germinated nodal buds were inoculated on the proliferation and subculture medium for multiplication and subculture. A total of 10 germinated nodal buds were inoculated for each treatment, and repeated 3 times. Using MS medium as the basic medium for proliferation and subculture hormone screening, the cytokinin is 6-BA, and the concentration range is (0.1mg / L, 0.5mg / L, 0.8mg / L, 1.0mg / L, 1.2 mg / L, 1.5mg / L, 6 concentration gradients), the auxin concentrations were (0.1mg / L, 0.5mg / L, 1.0mg / L, 3 concentration gradients), using the uniform design method, the results are shown in Table 2.

[0058] Table 2 Proliferation medium hormone screening

[0059]

[0060] The proliferation and subculture medium is MS+1.0mg / L 6-BA+0.5mg / L IAA+sucrose 30g / L+agar 5g / L, pH value 5.8, light-dark cycle is 10:14, light intensity is 1500Lux, The temperature is 23-28° C. After 60 day...

Embodiment 3

[0062] A method for rapid propagation of magnificent miraculous seeds, comprising the following steps:

[0063] The proliferating seedlings prepared in Example 2 were inoculated onto the rooting medium, and the formulation of the rooting medium was shown in 4 formulations in Table 3, and the rooting medium was screened.

[0064] Table 3 Rooting Medium Hormone Screening Results

[0065]

[0066] The results are shown in Table 3. MS medium + 1.0mg / L IAA + 1.0mg / L IBA + sucrose 30g / L + agar 5g / L, pH 5.8 is the best formula, and the culture period is 30 days. Compared with other formulas, it has significant advantages, and the rooting rate reaches 93%. %.

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Abstract

The invention provides a method for aseptic germination and rapid propagation of Michelia majestic seeds, belonging to the technical field of plant tissue culture. In the present invention, the mature seeds of Michelia majestic are used as explants, and through the steps of explant disinfection, aseptic germination, multiplication and rooting culture steps, artificial propagation, introduction and domestication, ex-situ protection, close-in-situ protection and in-situ protection of Michelia majestic are carried out. etc., and effectively avoid the natural disappearance and extinction of the wild resources of Magnificent Michelia, the expansion of the population in nature and its development and utilization in gardens and landscapes. According to the method provided by the invention, the seeds start to germinate in 10 days, the aseptic germination rate of the seeds is 83%, the reproduction cycle is 60 days, the multiplication coefficient is 5.1, the rooting rate is 93%, and the transplanting survival rate is 96%, which greatly improves the growth rate of Magnificent Miracles. The reproduction number and growth rate provide technical support for the protection and breeding, introduction and domestication, preservation and large-scale production of this species.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for aseptic germination and rapid propagation of Michelia majestic seeds. Background technique [0002] Michelia lacei (Michelia lacei) belongs to the genus Michelia magnolia. Michelia lacei is a species endemic to Yunnan, China, with a narrow distribution area. The few wild populations found so far are mainly distributed in Yunnan. The magnificent smile has high ornamental and economic value. In recent years, due to the interference of human activities such as eating its seeds, opening up wasteland, and grazing, the natural renewal and rejuvenation of the population has been slow, and the population has continued to decline and is almost extinct. Magnificent Michelia has few fruits and seeds, and there are cases where the fruit is small and small, so it is difficult to reproduce and renew naturally. [0003] So far, there is no report on the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 罗桂芬孙卫邦葛佳陶丽丹蔡磊
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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