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A method for tissue culture and rapid propagation of an excellent single plant of hibiscus purple flowers in dry land

A technology for tissue culture, rapid propagation and hibiscus, applied in horticultural methods, botanical equipment and methods, culture medium, etc., can solve the problems of narrow distribution area of ​​hibiscus in dry land, difficulty in introduction and domestication, etc., and maintain genetic stability and consistency. , the reproduction steps are simplified, and the effect of improving the reproduction coefficient

Active Publication Date: 2020-02-21
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for tissue culture and rapid propagation of high-quality single-plant hibiscus purple flowers in dry land, to fill the gap in the biotechnology of hibiscus in dry land, and to solve the problem that hibiscus in dry land has a narrow distribution area in the wild and is difficult to introduce and domesticate

Method used

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  • A method for tissue culture and rapid propagation of an excellent single plant of hibiscus purple flowers in dry land
  • A method for tissue culture and rapid propagation of an excellent single plant of hibiscus purple flowers in dry land
  • A method for tissue culture and rapid propagation of an excellent single plant of hibiscus purple flowers in dry land

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Experimental program
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Effect test

Embodiment 1

[0034] 1. Media and matrix screening:

[0035] Take the young terminal buds and lateral buds of an excellent single plant of the dryland hibiscus purple flower system as explants, soak them in 1% soapy water for 10 minutes, rinse them with running water, put them on a super-clean table, and cut them into stems with single nodes section, disinfected with 75% alcohol for 10 seconds, rinsed with sterile water for 3 times, disinfected with 0.1% mercuric chloride solution for 5 minutes, washed with sterile water for 3 to 5 times, and inoculated in the prepared hibiscus upland induction medium. 1 section per bottle.

[0036] The stem segments were inoculated in MS+0.1mg / l 6-BA+0.5mg / L NAA, MS+0.5mg / l 6-BA+0.5mg / LNAA, MS+0.8mg / l 6-BA+0.5mg / LNAA, MS+1mg / l 6-BA+0.5mg / LNAA, MS+2mg / l 6-BA+0.5mg / LNAA medium, sucrose 30g / L, agar 5g / L, pH5.8. The light intensity is 1000LUX, the temperature is 23-30°C, and the light time is 10 hours. The statistical induction rate is as follows:

[0037...

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Abstract

The invention discloses a Hibiscus aridicola Anthony purple flower excellent single plant tissue culture rapid propagation method. Hibiscus aridicola Anthony purple flower excellent single plants areadopted as explants, and through a series of culture steps of explant sterilixation, induction, differentiation, proliferation and rooting, the bottle neck problems of tissue culture rapid propagation, introduction and domestication and commercial development and application of Hibiscus aridicola Anthony are effectively solved, the situations that natural plants are reduced and natural vegetationregions are damaged because of over-use of wild resources are effectively avoided, and particularly the method has the beneficial effect of maintaining stability of special shapes of excellent singleplants. The method has a very good development and application prospect. By adopting the method, the induction differentiation rate is 80%, the breeding cycle is 30 days, the growth coefficient is 4,the rooting rate is 95%, the transplanting survival rate is 92%, the reproduction number and the growth velocity of the Hibiscus aridicola Anthony are greatly increased, and technical supports for introduction and domestication, preservation and on-scale production of the specimen are provided.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of plants in biotechnology, in particular to a method for tissue culture and rapid propagation of an excellent single plant of hibiscus purple flowers in dry land. Background technique [0002] Dryland Hibiscus Hibiscus aridicola Anthony belongs to the Malvaceae Hibiscus genus, a beautiful multi-flowered shrub. Dryland hibiscus is a unique species in the dry, hot, dry and warm valley of the Jinsha River. It is only distributed in Lijiang, Yunnan and Yanbian, Sichuan, in dry, hot, dry and warm valleys at an altitude of 600-2,500 meters. White flowers are more common, and purple and pink flowers are extremely rare. At the same time, due to the fact that there are less than 5 natural distribution points, the population continues to decline. In 2004, it was listed as an endangered species in the "Red List of Species in China" [EN B2ab(ii)]. In recent years, due to the construction...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G24/60
CPCA01H4/001A01H4/008
Inventor 罗桂芬陈高孙卫邦
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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