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A method for aseptic germination and rapid propagation of hibiscus seeds in dry land

A technology for seeds and hibiscus, which is applied in horticultural methods, seed immunization, seed and rhizome treatment, etc., can solve the problems of narrow distribution area of ​​hibiscus in dry land, difficulty in introduction and domestication, etc., and achieves restoration of genetic diversity, simplified reproduction steps, and seedling formation. easy effect

Active Publication Date: 2020-04-21
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for aseptic germination and rapid propagation of hibiscus seeds in dry land, to fill the gap in the biological technology of hibiscus in dry land, and to solve the problem that hibiscus in dry land has a narrow distribution area in the wild and is difficult to introduce and domesticate

Method used

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  • A method for aseptic germination and rapid propagation of hibiscus seeds in dry land
  • A method for aseptic germination and rapid propagation of hibiscus seeds in dry land
  • A method for aseptic germination and rapid propagation of hibiscus seeds in dry land

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Embodiment 1

[0037] 1. Media and matrix screening:

[0038] Take the plump and slightly yellowed fruit of Hibiscus aridum as explants, soak it in 1% soapy water for 10 minutes, rinse it with running water, put it on an ultra-clean table, disinfect it with 75% alcohol for 15 minutes, and rinse it with sterile water for 3 times , remove the peel, take out the seeds, sterilize the surface with 0.1% mercuric chloride solution for 5 minutes, wash with sterile water for 3 to 5 times, inoculate in the prepared hibiscus upland seed germination medium, 5 capsules per bottle.

[0039]The seeds were inoculated on MS, 1 / 2MS, 1 / 3MS, and modified MS (close to 1 / 4MS) medium, each medium did not contain organic matter and hormones, sucrose 30g / L, agar 5g / L, pH5.8 . After 7 days in dark culture medium and 13 days in light culture, the germination rate of seeds was counted. The light intensity is 1000LUX, and the temperature is 23-30°C.

[0040] Table 1 Screening of seed aseptic germination medium

[00...

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Abstract

The invention discloses an aseptic germinating and rapid propagating method for hibiscus aridicola anthony seeds. According to the aseptic germinating and rapid propagating method for the hibiscus aridicola anthony seeds, by taking mature hibiscus aridicola anthony seeds as explants, and performing a series of cultivation steps of sterilizing the explants, performing aseptic germinating on the seeds, proliferating and rooting, the problems about artificial reproduction, introduction and domestication, and development and utilization of the hibiscus aridicola anthony in landscape gardening areeffectively solved, and the problems that the number of natural plants is reduced and a natural vegetation area is destructed because of excessive utilization of wild resources are effectively avoided; the aseptic germinating and rapid propagating method for the hibiscus aridicola anthony seeds has important significance in the aspect of development and utilization. According to the method provided by the invention, the seeds start to germinate within 20 days; the aseptic germinating rate is 60 percent; the reproduction period is 30 days; the proliferation coefficient is 5; the rooting rate is98 percent; the transplanting survival rate is 95 percent; the proliferation number and the growth rate of the hibiscus aridicola anthony are greatly improved; technical support is provided for the introduction and domestication, gardening development, storage and scale production of species.

Description

technical field [0001] The invention relates to a method for aseptic germination and rapid propagation of plant seeds in biotechnology, in particular to a method for aseptic germination and rapid propagation of hibiscus seeds in dry land. Background technique [0002] Dryland Hibiscus Hibiscus aridicola Anthony is a beautiful multi-flowered small shrub belonging to the genus Hibiscus in the Malvaceae family. The dryland hibiscus is a unique species and a regional landmark species in the dry-hot valley of the Jinsha River. It is only distributed in Lijiang, Yunnan and Yanbian, Sichuan, in dry-hot and dry-warm valleys with an altitude of 600-2500 meters. Due to the fact that there are less than 5 distribution points, the population continues to decline, and it was listed as an endangered species in the "Red List of Species in China" in 2004 [EN B2ab(ii)]. In recent years, due to dam construction, habitat loss, and human activities, the living environment of this species has b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01C1/00A01C1/08A01G24/15A01G24/12A01G17/00
CPCA01C1/00A01C1/08A01G17/005A01H4/001A01H4/008
Inventor 罗桂芬陈高孙卫邦
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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