A kind of tissue culture rapid propagation method of Diantong
A technology for tissue culture and rapid propagation of T. sylvestris, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of lack of natural resources and inability to achieve a large number of T. sylvestris, and maintain genetic stability and consistency. , The effect of high effective reproduction rate and simplified reproduction steps
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[0033] Take the young terminal buds and side buds of Diantong as explants, soak them in 1% soapy water for 10 minutes, rinse them with running water, put them on a super-clean table, cut them into stems with single nodes, and use 75% Disinfect with alcohol for 10 seconds, rinse with sterile water for 3 times, disinfect the surface with 0.1% mercuric chloride solution for 5 minutes, wash with sterile water for 3 to 5 times, and inoculate in the prepared co-medium for induction and differentiation of Diantong, 1 per bottle Festival.
[0034] 1. The effect of different induction differentiation media on the induction rate
[0035]The stem segments were inoculated in the following medium: ①MS+0.1mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ②MS+0.5mg / L 6-BA+0.5 mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ③MS+0.8mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ④MS+1mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, pH5.8, ⑤MS+2mg / L 6-BA+0.5mg / L NAA+sucrose 30g / L+agar 5g / L, ...
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