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Manufacturing method for mucuna pruriens test-tube plant

A production method and test tube technology, applied in the field of plant tissue culture, can solve the problems of few species of test tube plants, cumbersome production process and high production cost, and achieve the effects of preventing external bacteria from entering the test tube, reducing production costs and preventing external bacteria from entering.

Active Publication Date: 2020-12-11
海南大学三亚南繁研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of fewer types of test-tube plants, higher production costs and cumbersome production processes, the present invention develops a method for making cat bean test-tube plants, which is specifically implemented according to the following steps:

Method used

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  • Manufacturing method for mucuna pruriens test-tube plant
  • Manufacturing method for mucuna pruriens test-tube plant
  • Manufacturing method for mucuna pruriens test-tube plant

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A preparation method of cat bean test-tube plant, comprising the following steps:

[0029] 1) Selection of materials: choose healthy seeds;

[0030] 2) Disinfection of seeds: Rinse the seeds with water for 20 minutes, then rinse 3 times with 1% detergent, then transfer to the aseptic workbench, soak in 75% alcohol for 2 minutes, rinse 5 times with sterile water, and then use Soak in 10% sodium hypochlorite for 10 minutes and rinse with sterile water 5 times;

[0031] 3) Seed inoculation and packaging: On a sterile workbench, inoculate the sterilized seeds into a transparent test tube and place it on the surface of the culture medium. Tie the mouth of the bottle tightly and seal it, and cut off the excess sealing film around it; the medium is MS medium, and each liter of medium also contains 70mL of baobab leaf extract, 0.05g of food coloring, and 0.5g of Shannong No. 1 mL, sucrose 30g, carrageenan 8g, pH 6.0;

[0032] 4) Seed culture: after inoculation, the seeds are...

Embodiment 2

[0036] A preparation method of cat bean test-tube plant, comprising the following steps:

[0037] 1) Selection of materials: choose healthy seeds;

[0038] 2) Disinfection of seeds: the seeds were first rinsed with water for 25 minutes, then rinsed 4 times with 2% detergent, then transferred to a sterile workbench, soaked in 70% alcohol for 1 minute, rinsed 6 times with sterile water, and then rinsed with sterile water. Soak in 10% sodium hypochlorite for 12 minutes and rinse with sterile water 4 times;

[0039] 3) Seed inoculation and packaging: On a sterile workbench, inoculate the sterilized seeds into a transparent test tube and place it on the surface of the culture medium. Tie the mouth of the bottle tightly and seal it, and cut off the excess sealing film around it; the medium is MS medium, and each liter of medium also contains 70mL of baobab leaf extract, 0.05g of food coloring, and 0.5g of Shannong No. 1 mL, sucrose 30g, carrageenan 8g, pH 5.8;

[0040] 4) Seed cu...

Embodiment 3

[0044] The difference between embodiment 3 and embodiment 1 is:

[0045] The baobab leaf extract is obtained by washing the fresh leaves of baobab with water, adding water for pulverization, filtering, centrifuging the filtrate at 2000rpm for 3 minutes to obtain a supernatant, and concentrating the supernatant to 50% of the original volume. % income.

[0046] The seed culture is that the seeds are inoculated and placed in a culture room, and cultured at a temperature of 18° C. and a light condition of 1800 lux.

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Abstract

The invention provides a manufacturing method for a mucuna pruriens test-tube plant. According to the manufacturing method, seeds of mucuna pruriens are taken as explants, the disinfected seeds are inoculated into a test tube containing a culture medium, the test tube is packaged, and then the mucuna pruriens test-tube plant is formed. According to the manufacturing method, the early-stage cultureprocess of the test-tube plant is omitted, a baobab leaf extract is used in the culture medium for replacing a growth regulator, the ornamental value of the test-tube plant is improved through ediblepigments, thus the pollution rate is decreased through the Shannon No.1, and the ornamental period of the test-tube plant is prolonged; the combined action of the baobab leaf extract, the edible pigments and the Shannon No.1 can prolong the optimal ornamental period of test-tube plant, the baobab leaf extract and the edible pigments have a synergistic antibacterial effect, thus the production cost is greatly reduced, market promotion is easy, and economic benefits are increased; and according to a test-tube plant product, the growth and development processes of the test-tube plant from seedsto plantlets can be observed continuously and clearly, and the test-tube plant product has good ornamental value and science popularization education value.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for preparing cat bean test-tube plants. Background technique [0002] With the improvement of people's living standards, people's aesthetic tastes have changed, and the original flower varieties can no longer meet people's needs. With the maturity of test-tube tissue culture technology and the improvement of modernization, countries around the world have launched extensive research on flower biotechnology, which has injected great vitality into flower production. [0003] Test-tube plants, also known as test-tube flowers, are a general term for miniature plants that grow in special transparent test tubes by inoculating explants into special transparent test tubes using plant tissue culture technology under artificially controlled sterile environment conditions. The beautifully shaped test tube provides young plants with a relatively closed growth space and medium, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/08A01G13/02
CPCA01H4/001A01H4/005A01H4/008A01C1/08A01G13/02
Inventor 李宏杨刘扬陈冠铭任杰李可贵
Owner 海南大学三亚南繁研究院
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