A SNP Molecular Marker Affecting Alpine Merino Wool Fiber Diameter and Its Application
A merino sheep and molecular marker technology, applied in the field of genetic engineering, can solve the problems that important genetic resources have not been effectively excavated and utilized, and the molecular marker system of fine-haired wool has not been established, so as to shorten the breeding cycle and improve the quality of selection. Breeding accuracy and the effect of reducing breeding costs
Active Publication Date: 2021-05-25
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology
In the past 50 years, fine-wool sheep genetics and breeding experts at home and abroad have been devoted to the research of candidate genes or molecular markers controlling wool traits, but so far no molecular marker system for fine-wool traits has been established, and a large number of important genetic resources for regulating wool traits are still available. Not being exploited and utilized effectively
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Embodiment 1
[0040] Example 1 Correlation Between Different Genotypes of Alpine Merino Sheep and Wool Fiber Diameter
[0041] 1 material
[0042] 1.1 Sample collection
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Abstract
The invention relates to the technical field of genetic engineering, in particular to a SNP molecular marker affecting the fiber diameter of Alpine Merino sheep and its application. The SNP molecular marker affecting the fiber diameter of Alpine Merino sheep is located in the International Sheep Reference Genome A / G mutation at the 44288858th nucleotide site on chromosome 7 of Oar_v4.0 version. The present invention also provides a pair of specific primers for identifying the above-mentioned SNP molecular markers and a kit containing the pair of specific primers, which can be used for early selection of superfine strains of Alpine Merino sheep, improve the accuracy of selection, and shorten the cultivation period , Accelerate the cultivation process. Moreover, the kit has the advantages of simple operation, reduces the breeding time for the excellent traits of Alpine Merino wool fiber diameter, reduces the breeding cost, shortens the cultivation period, and accelerates the cultivation process, and has high application value.
Description
technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a SNP molecular marker affecting the fiber diameter of Alpine Merino sheep and its application. Background technique [0002] The new breed of alpine Merino sheep is the world's first new breed of wool and meat Merino sheep that can adapt to the alpine cold and drought ecological zone at an altitude of 2400-4070m and the wool fiber diameter is 19.1-21.5μm. Excellent germplasm resources are provided. The wool yield and fiber fineness quality of fine-wool sheep are important indicators to determine its economic value, and the objective inspection of wool quality is getting more and more attention in wool sales, and it is more and more combined with textile performance, breeding and production. Tightness, wool fiber diameter is the most important test index in wool fiber inspection, almost all properties of wool are related to or directly determined by fiber diameter. ...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 袁超郭婷婷卢曾奎刘建斌岳耀敬牛春娥杨博辉孙晓萍李建烨
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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