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Application of ganoderic acid A to preparation of drugs for activating brain CD4+T cells and inhibiting neuroinflammation

A technology of cell inhibition and neuroinflammation, applied in the field of biomedicine, can solve the problem that the application research of ganoderma acid A is still less

Inactive Publication Date: 2020-11-27
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the research on Ganoderma lucidum at home and abroad mainly focuses on Ganoderma lucidum polysaccharides, triterpenes anti-tumor and improving immunity, but there are few studies on the application of Ganoderma acid A in activating brain CD4+ T cells to inhibit neuroinflammation

Method used

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  • Application of ganoderic acid A to preparation of drugs for activating brain CD4+T cells and inhibiting neuroinflammation
  • Application of ganoderic acid A to preparation of drugs for activating brain CD4+T cells and inhibiting neuroinflammation
  • Application of ganoderic acid A to preparation of drugs for activating brain CD4+T cells and inhibiting neuroinflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Protective effect of ganoderma acid A on LPS-induced BV2 cell injury model

[0018] According to the cell culture method, use DMEM high glucose medium containing 10% fetal bovine serum (FBS) plus penicillin 100units / ml, streptomycin 100ug / ml, place at 37 ° C, 5% CO 2 Cultured in an incubator, the culture medium was changed every 24 hours, and the cells were passaged once every 48 hours. Take BV2 cells in the logarithmic growth phase, plant them in 12-well plates, add 1ml of medium containing 2μg / ml LPS after 12h, incubate for 4h, and then directly add 1ml of medium containing ganoderic acid A for treatment for 24h, each treatment Three replicates were used to detect the cell survival rate by MTT method, and the levels of L-1β, IL-6, TNF-α, NF-κB, etc. in the cells were measured by Western boltiong method. The results are shown in Table 1 and Table 2.

[0019] Table 1 Protective effect of ganoderma acid A on LPS-induced BV2 cell injury model

[0020]

[0...

Embodiment 2

[0027] Example 2: Experimental research on activation of CD4+ T cells in the brain of APP / PS1 double transgenic mice by Ganoderma acid A (abbreviated as GA)

[0028] The experiment was carried out with SPF grade 2-month-old APP / PS1, and the experimental settings were: normal control group, model group, and treatment group (GA25mg / kg body weight, GA 50mg / kg body weight), with 8 mice in each group. The normal control group and the model group were intragastrically administered with 2 mL / d normal saline, and the rest of the treatment groups (GA 25 mg / kg body weight, GA 50 mg / kg body weight) were given ganoderma acid A treatment according to their respective doses (gastric administration volume 2 mL / d ), and each group was continuously administered for 6 months. After 6 months, after behavioral evaluation, Western boltiong test was used to detect L-1β, IL-6, TNF-α, NF-κB, Aβ in brain tissue 1-42 , p-Tau, APOE, IBA-1 and GFAP protein expression.

[0029] Table 3 Effects of ganode...

Embodiment 3

[0038] Example 3: Experimental research on the activation of CD4+ T cells in the brain of SAMP8 rapidly aging mice by Ganoderma acid A (abbreviated as GA)

[0039] Two-month-old SAMP8 mice of SPF grade were used as research objects. Experimental settings: normal control group, model group, treatment group (GA 25 mg / kg body weight, GA 50 mg / kg body weight), 8 mice in each group. The control group and the model group were intragastrically administered with 2mL / d normal saline, and the remaining treatment groups (GA 25mg / kg, GA 50mg / kg) were intragastrically administered ganocid A according to their respective doses, and each group continued the administration for 6 months. Six months later, after the behavioral evaluation, the expressions of L-1β, IL-6, TNF-α, NF-κB, IBA-1 and GFAP in brain tissue were detected by Western boltiong experiment.

[0040] Table 5 Effect of ganoderma acid A on CD4+T, microglia and astrocytes in the brain of SAMP8 rapidly aging mice

[0041]

[0...

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Abstract

The invention discloses an application of ganoderic acid A to preparation of drugs for activating brain CD4+T cells and inhibiting neuroinflammation. Experiments show that the ganoderic acid A significantly up-regulates the content of CD4+T in mouse brains, reduces the levels of L-1beta, IL-6, TNF-alpha, NF-kB and the like, and down-regulates expression of Abeta1-42, p-Tau, APOE, IBA-1, and GFAP,which suggests that the ganoderic acid A can activate the CD4+T cells in brains and inhibit neuroinflammation. Acute toxicity experiments on cells and animals show that the safe dose range of the ganoderic acid A is wide, and can be applied to preparation of the drugs for activating the CD4+T cells in brains and inhibiting neuroinflammation.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of ganoderma acid A in the preparation of drugs for activating brain CD4+ T cells and inhibiting neuroinflammation. Background technique: [0002] Neuroinflammation occurs in the central nervous system (CNS) as a result of infection, injury, autoimmune processes or toxicity. Microglia, as specifically resident macrophages in the central nervous system, play a key role in the onset and resolution of neuroinflammation, however, their effects on neurons are complex and phenotypic plasticity of microglia can be directly affected by regions of the brain microenvironment, therefore, the dichotomous M1 / M2 markers used to describe the activity of other macrophages are insufficient to reflect the true heterogeneity of the microglial population, which is known to Glial cells may exhibit a "disease-associated microglial signature" associated with the expression of ge...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61P25/00A61P29/00
CPCA61K31/575A61P25/00A61P29/00
Inventor 王东东陈地灵郭殷锐祁龙凯唐晓萃谢意珍李良秋吴清平
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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