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Extraction method of cannabis sativa polysaccharide and product and application of cannabis sativa polysaccharide

An extraction method, the technology of hemp polysaccharides, applied in the field of biochemical engineering, can solve the problems of not being reused, environmental pollution, resource waste, etc., and achieve the effect of simple and easy-to-operate extraction method, high purity, and scavenging DPPH free radicals

Active Publication Date: 2020-11-24
YUNNAN HEMPMON PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Industrial hemp flowers and leaves contain a large amount of plant polysaccharides, which are rarely developed and utilized at present. After using hemp flowers and leaves to extract cannabinoid active ingredients such as CBD, the residues are often discarded and not reused, causing environmental pollution and resource pollution. waste

Method used

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  • Extraction method of cannabis sativa polysaccharide and product and application of cannabis sativa polysaccharide
  • Extraction method of cannabis sativa polysaccharide and product and application of cannabis sativa polysaccharide
  • Extraction method of cannabis sativa polysaccharide and product and application of cannabis sativa polysaccharide

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Embodiment 1

[0064] The present embodiment provides a kind of extraction method of cannabis polysaccharide, described method is as follows:

[0065] (1) the residue raw material after 1kg hemp leaf is extracted CBD is soaked in water, and the mass ratio of raw material and aqueous solution is 1:12;

[0066] (2) The raw material soaked in step (1) is placed in a circulating ultrasonic extraction machine for ultrasonic stirring extraction in an aqueous solution, the power is 1200W, the duty ratio is 1:1, the time is 2h, the temperature is 30 ° C, and the pH of the aqueous solution is The value is 6;

[0067] (3) step (2) after the extraction is finished, first perform coarse filtration and then fine filtration, the coarse filtration adopts a drum-type belt filter filtration device, and the fine filtration adopts a disc centrifuge to filter, and then uses a microfiltration membrane with a molecular weight cut-off of 300KDa to remove The filtrate was concentrated under reduced pressure and co...

Embodiment 2

[0071] The present embodiment provides a kind of extraction method of cannabis polysaccharide, described method is as follows:

[0072] (1) 1kg dry cannabis flower raw material is soaked in water, and the mass ratio of the raw material and the aqueous solution is 1:30;

[0073] (2) placing the raw material soaked in step (1) in a heating extraction tank and extracting in an aqueous solution, the temperature is 80 ° C, the pH value of the aqueous solution is 7, and 0.1% of cellulase is added in addition;

[0074] (3) step (2) after the extraction is finished, first perform coarse filtration and then fine filtration, the coarse filtration adopts a drum-type belt filter filtration device, and the fine filtration adopts a disc centrifuge to filter, and then uses a microfiltration membrane with a molecular weight cut-off of 300KDa to remove The filtrate is concentrated by ultrafiltration membrane and concentrated to 1 / 4 of the filtrate volume;

[0075] (4) The concentrated solutio...

Embodiment 3

[0078] The present embodiment provides a kind of extraction method of cannabis polysaccharide, described method is as follows:

[0079] (1) 1kg of fresh hemp leaf raw material is soaked in water, and the mass ratio of raw material and aqueous solution is 1:5;

[0080] (2) The raw material soaked in step (1) is placed in a circulating ultrasonic extraction machine for ultrasonic stirring extraction in an aqueous solution, the power is 1000W, the duty ratio is 1:3, the time is 2h, the temperature is 45 ° C, and the pH of the aqueous solution is The value is 5; additionally add the mixture of cellulase, pectinase, β-glucanase and xylanase in equal proportion, and the enzyme concentration is 0.5%;

[0081] (3) step (2) after the extraction is finished, first perform coarse filtration and then fine filtration, the coarse filtration adopts a drum-type belt filter filtration device, and the fine filtration adopts a disc centrifuge to filter, and then uses a microfiltration membrane w...

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Abstract

The invention relates to an extraction method of cannabis sativa polysaccharide and a product and application of cannabis sativa polysaccharide. The extraction method comprises the following steps: soaking a cannabis sativa raw material, then carrying out extracting, carrying out solid-liquid separation, concentrating filtrate, and precipitating the concentrated solution in alcohol to obtain the cannabis sativa polysaccharide. The invention further provides a purification method of the cannabis sativa polysaccharide. The extraction, separation and purification methods provided by the inventionare simple and easy to operate; and the prepared cannabis sativa polysaccharide is high in purity, has remarkable effects of removing DPPH free radicals, resisting oxidation, whitening skin, resisting aging, improving immunity, regulating blood fat, resisting tumors and the like, and can be widely applied to cosmetics, health care products, foods or medicines. Meanwhile, the raw materials of theextraction method comprise residues obtained after CBD and other cannabinoid active ingredients are extracted from cannabis sativa flowers and leaves, so that the extraction method also provides a newstrategy for recycling the residues obtained after the CBD and other cannabinoid active ingredients are extracted from the cannabis sativa flowers and leaves, waste is turned into wealth, and high-value recycling of resources is realized.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a method for extracting hemp polysaccharides and its products and applications. Background technique [0002] Industrial hemp (scientific name: Cannabis sativa L.) is a plant of the family Moraceae and the genus Cannabis. The content of tetrahydrocannabinol (THC) in the flowers and leaves during the growth period is less than three per thousand. It does not have the value of extracting THC or directly use it as Drug smoking can be legally carried out in large-scale planting and industrial development and utilization, and its economic and medicinal value are extremely high. As early as 3,500 years ago, there was a record of cannabis cultivation in my country. Hemp has a wide range of uses. The hemp bark can be peeled to make fiber weaving, papermaking, and ropes. The seeds can be eaten and oil-pressed. The flowers and leaves can be used as medicine. Th...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 赵兵赵庆生王培东柳旭常坦然于朝晖
Owner YUNNAN HEMPMON PHARMA CO LTD
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