A kit for monitoring the activity of signaling pathways in vivo

A technology of signaling pathways and kits, applied in the field of biomedicine, can solve problems such as the inability to realize real-time dynamic monitoring of the activity of signal pathways, and achieve the effect of dynamic monitoring

Active Publication Date: 2022-07-01
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] These methods first extract target molecules from cells or tissues, and then conduct in vitro detection, which only reflect the concentration of target molecules and the activity of signaling pathways at a certain moment, and cannot realize real-time dynamic monitoring of the activity of signaling pathways in vivo

Method used

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  • A kit for monitoring the activity of signaling pathways in vivo

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Embodiment Construction

[0024] In this embodiment, the light-emitting element is ZnGa 2 O 4 , the electron conduction element is a ferrocene derivative, and the recognition element is DNA.

[0025] ZnGa 2 O 4 Preparation of nanoparticles:

[0026] Synthesis of ZnGa in Oil Phase 2 O 4 Nanoparticles: First, 4.5 g of octadecanoic acid and 0.6 g of NaOH were weighed and dissolved in a mixed solution containing 8 mL of deionized water and 18 mL of ethanol. Then, Zn(NO) was added sequentially under vigorous stirring. 3 ) 2 (2.0M, 0.5mL), Ga(NO 3 ) 2 (0.4M, 5.0mL) and Cr(NO 3 ) 2 (8mM, 625μL). Sodium hydroxide was added to adjust the pH to 10.0. The resulting mixture was stirred at room temperature for 20 minutes. The solution was transferred to a polytetrafluoroethylene-lined reaction kettle, and after reacting at 220 °C for 10 h, the prepared ZnGa was collected by centrifugation. 2 O 4 Nanoparticles were washed 3 times with a mixture of cyclohexane:ethanol=1:1.

[0027] Preparation of fer...

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Abstract

The invention discloses a kit for monitoring the activity of a signal pathway in vivo, which is composed of an electron transfer monitoring probe and a living body imager. The living body imager is wirelessly connected with the electron transfer monitoring probe to detect its fluorescence intensity. The electron transfer monitoring probe It includes a light-emitting element, an electron-conducting element and an identification element, the light-emitting element and the electron-conducting element are combined by hydrophobic force, and the electron-conducting element and the identification element are combined by a phosphodiester bond. The electron transfer monitoring probe of the present invention can be used for in vivo real-time dynamic monitoring of the activity of the signal pathway, and the identification element is a nucleic acid aptamer and other substances with targeted identification capability, which can realize real-time dynamic identification of target molecules in vivo.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a kit for monitoring the activity of signaling pathways in vivo. Background technique [0002] A signaling pathway refers to the process by which chemical or physical signals are transmitted within a cell through a series of molecular events. Various physiological activities such as cell growth, proliferation, metabolism, and migration need to be achieved through signaling pathways, and the destruction or overexpression of signaling pathways can easily lead to various diseases including cancer. For example, overexpression of Rho / ROCK signaling pathway can easily lead to the invasion and metastasis of tumor cells such as lung cancer, liver cancer, gastric cancer and abdominal aneurysm; overexpression of BDNF / TrkB signaling pathway can promote the growth, proliferation, survival and migration of cancer cells; ERK signaling pathway Overexpression disrupts the blood-brain barr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/00
CPCA61B5/0071A61B2503/40A61B2503/42
Inventor 袁荃谈洁
Owner HUNAN UNIV
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