Kit for monitoring activity degree of in-vivo signal path

A signal pathway and kit technology, applied in the field of biomedicine, can solve problems such as the inability to realize real-time dynamic monitoring of the activity of signal pathways, and achieve the effect of dynamic monitoring

Active Publication Date: 2020-11-20
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] These methods first extract target molecules from cells or tissues, and then conduct in vitro detection, which only reflect the concentration of target molecules and the activity of signaling pathways at a certain moment, and cannot realize real-time dynamic monitoring of the activity of signaling pathways in vivo

Method used

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  • Kit for monitoring activity degree of in-vivo signal path

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Embodiment Construction

[0024] In this embodiment, the light-emitting element is ZnGa 2 o 4 , the electron conduction element is a ferrocene derivative, and the recognition element is DNA.

[0025] ZnGa 2 o 4 Preparation of nanoparticles:

[0026] Synthesis of ZnGa in oil phase 2 o 4 Nanoparticles: first weigh 4.5g octadecanoic acid and 0.6g NaOH and dissolve in a mixed solution containing 8mL deionized water and 18mL ethanol. Then add Zn(NO 3 ) 2 (2.0M,0.5mL), Ga(NO 3 ) 2 (0.4M,5.0mL) and Cr(NO 3 ) 2 (8mM, 625μL). Sodium hydroxide was added to adjust the pH to 10.0. The resulting mixture was stirred at room temperature for 20 minutes. Transfer the solution to a polytetrafluoroethylene-lined reactor, react at 220 °C for 10 h, and centrifuge to collect the prepared ZnGa 2 o 4 Nanoparticles were washed 3 times with a mixture of cyclohexane:ethanol=1:1.

[0027] Preparation of ferrocene derivatives [Fe]:

[0028] Commercially available (S)-3-amino-1,2-propanediol was coupled with ferro...

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Abstract

The invention discloses a kit for monitoring the activity degree of an in-vivo signal path. The kit consists of an electron transfer monitoring probe and a living body imager, wherein the living bodyimager is wirelessly connected with the electron transfer monitoring probe to detect the fluorescence intensity, the electron transfer monitoring probe comprises a light-emitting element, an electronconduction element and an identification element, the light-emitting element is combined with the electron conduction element through a hydrophobic acting force, and the electron conduction element iscombined with the identification element through a phosphodiester bond. The electron transfer monitoring probe can be used for in-vivo real-time dynamic monitoring of the activity degree of a signalpath, the recognition element is a nucleic acid aptamer and other substances with the targeting recognition capability, and real-time dynamic recognition of in-vivo target molecules can be achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a kit for monitoring the activity of signal pathways in vivo. Background technique [0002] Signaling pathways refer to the process by which chemical or physical signals are transmitted within cells through a series of molecular events. Various physiological activities such as cell growth, proliferation, metabolism, and migration need to be realized through signaling pathways, and the destruction or overexpression of signaling pathways can easily lead to various diseases including cancer. For example, overexpression of Rho / ROCK signaling pathway can easily lead to the invasion and metastasis of tumor cells such as lung cancer, liver cancer, gastric cancer and abdominal aneurysm; overexpression of BDNF / TrkB signaling pathway can promote the growth, proliferation, survival and migration of cancer cells; ERK signaling pathway Overexpression can disrupt the blood-brain barrier and cause b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
CPCA61B5/0071A61B2503/40A61B2503/42
Inventor 袁荃谈洁夏杭
Owner HUNAN UNIV
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