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Series of culture kits for regenerating hedychium coronarium hookeriana plants and application thereof

The technology of Huke ginger flower and kit, which is applied in the field of plant tissue culture, can solve the problems such as the difference in the concentration ratio of plant growth regulators, and achieve the effect of simplified disinfection operation steps, simple operation steps and high regeneration efficiency

Active Publication Date: 2020-11-17
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these technical schemes, the types of plant growth regulators selected and their concentration ratios are very different among different plants.

Method used

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  • Series of culture kits for regenerating hedychium coronarium hookeriana plants and application thereof
  • Series of culture kits for regenerating hedychium coronarium hookeriana plants and application thereof
  • Series of culture kits for regenerating hedychium coronarium hookeriana plants and application thereof

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Comparison scheme
Effect test

Embodiment 1

[0037] The present invention is used for the serial culture kit of regenerated Huke ginger plant and an embodiment of its application, the present embodiment is used for the serial culture kit of regeneration Huke ginger plant, comprises seed germination medium, callus Induction medium, callus proliferation medium and callus redifferentiation medium.

[0038] In the seed germination medium, the concentration of sucrose is 28g / L, and the concentration of agar is 7g / L; in the callus induction medium, the concentration of kinetin is 0.1mg / L, 2,4-dichloro The concentration of phenoxyacetic acid is 0.5mg / L, the concentration of sucrose is 28g / L, and the concentration of agar is 7g / L; In the described callus proliferation medium, the concentration of kinetin is 0.1mg / L, 2,4 -The concentration of dichlorophenoxyacetic acid is 0.3mg / L, the concentration of sucrose is 28g / L, and the concentration of agar is 7g / L; In the described callus redifferentiation medium, the concentration of 6-...

Embodiment 2

[0047] The present invention is used for the serial culture kit of regenerated Huke ginger plant and an embodiment of its application, this embodiment is used for the serial culture kit of regeneration Huke ginger plant, comprises seed germination medium, callus Induction medium, callus proliferation medium and callus redifferentiation medium.

[0048] In the seed germination medium, the concentration of sucrose is 29g / L, the concentration of agar is 7.5g / L; in the callus induction medium, the concentration of kinetin is 0.3mg / L, 2,4-di The concentration of chlorophenoxyacetic acid is 0.8mg / L, and the concentration of sucrose is 29g / L, and the concentration of agar is 7.5g / L; In described callus proliferation medium, the concentration of kinetin is 0.15mg / L, 2 , the concentration of 4-dichlorophenoxyacetic acid is 0.4mg / L, the concentration of sucrose is 29g / L, and the concentration of agar is 7.5g / L; In the described callus redifferentiation medium, the content of 6-benzylami...

Embodiment 3

[0051] The present invention is used for the serial culture kit of regenerated Huke ginger plant and an embodiment of its application, this embodiment is used for the serial culture kit of regeneration Huke ginger plant, comprises seed germination medium, callus Induction medium, callus proliferation medium and callus redifferentiation medium.

[0052] In the seed germination medium, the concentration of sucrose is 30g / L, and the concentration of agar is 8g / L; in the callus induction medium, the concentration of kinetin is 0.5mg / L, 2,4-dichloro The concentration of phenoxyacetic acid is 1.0mg / L, the concentration of sucrose is 30g / L, and the concentration of agar is 8g / L; In the described callus proliferation medium, the concentration of kinetin is 0.2mg / L, 2,4 -The concentration of dichlorophenoxyacetic acid is 0.5mg / L, the concentration of sucrose is 30g / L, and the concentration of agar is 8g / L; In the described callus redifferentiation medium, the concentration of 6-benzyla...

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Abstract

The invention relates to a series of culture kits for regenerating hedychium coronarium hookeriana plants and an application thereof, and belongs to the field of plant tissue culture. The series of culture kits for the regenerating hedychium coronarium hookeriana plants comprises a seed germination culture medium, a callus induction culture medium, a callus proliferation culture medium and a callus redifferentiation culture medium. A hedychium coronarium hookeriana callus regenerating system is simple in operation steps, low in explant pollution rate, high in callus induction speed, high in regenerating efficiency, free of seedling strengthening and rooting steps and capable of achieving one-step seedling formation.

Description

technical field [0001] The invention relates to a serial culture kit for regenerating Huke ginger flower plants and an application thereof, belonging to the field of plant tissue culture. Background technique [0002] Compared with other plants in the same genus, Huke ginger flower has a short plant, broad leaves, light yellow flower color and black aril, which has important research, development and utilization value. The plant callus regeneration system is the basic technology platform for further genetic manipulation and breeding improvement of the plant. At present, in the genus Zingiber officinalis, the types that have established callus regeneration systems include white ginger and red ginger. These two species belong to the types with thick rhizomes and thin lateral roots, which are distributed at middle and low altitudes, while Huke Jianghua has small rhizomes, thick lateral roots, and is distributed at high altitudes, and the species are quite different. In the se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李永泉胡秀张媚谭嘉川
Owner ZHONGKAI UNIV OF AGRI & ENG
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