Method for debonding sticky fertilized eggs of marine fishes
A fertilized egg, seawater technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of low hatching rate, incompleteness, sticky eggs easy to agglomerate, etc.
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Embodiment 1
[0020] Taking the viscous fertilized eggs of redfin puffer as an example, they were debonded with tannic acid seawater solution.
[0021] Step 1: preparing a debonding solution; using seawater as a solvent and tannic acid as a solute, preparing a 0.05% tannic acid seawater solution;
[0022] Step 2: aerating and immersing; immersing the fertilized eggs in the tannic acid seawater solution, inflating the water at the same time, fully immersing for 30 seconds;
[0023] Step 3: cleaning and counting; take out the fertilized eggs after soaking, place them in clean seawater for cleaning, and obtain debonded fertilized eggs, then put the debonded fertilized eggs into a petri dish, add clean seawater , let stand for 10min, pour off the seawater, count, and calculate the peeling rate.
[0024] The debonded fertilized eggs were microscopically observed under a dissecting microscope, and then the debonded fertilized eggs were hatched: 200 fertilized eggs were placed in clean seawater i...
Embodiment 2
[0026] Taking the viscous fertilized eggs of puffer puffer as an example, they were debonded with tannic acid seawater solution.
[0027] The mass concentration of the tannic acid seawater solution used is 0.05%, and the time for aerated soaking is 20s. Other operations are the same as in Example 1.
Embodiment 3
[0029] Taking the viscous fertilized eggs of puffer puffer as an example, they were debonded with tannic acid seawater solution.
[0030] The mass concentration of the tannic acid seawater solution used is 0.05%, and the time for aerated soaking is 20s. Other operations are the same as in Example 1.
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