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Preparation method of rat myocardial ischemia reperfusion model

A technology of myocardial ischemia and reperfusion, which is applied in the fields of animal restraint equipment, medical science, veterinary equipment, etc., can solve the problems of high preparation cost and poor perfusion effect, and achieve long survival time, ready perfusion effect and easy observation effect of effect

Inactive Publication Date: 2020-11-10
THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a preparation method of a rat myocardial ischemia-reperfusion model, which solves the problems of poor perfusion effect and high preparation cost in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The invention provides a technical scheme: a method for preparing a rat myocardial ischemia-reperfusion model, comprising the following steps:

[0025] S1. Surgical equipment needed for the preparation of the experiment: 10% water and 1ml syringe, iodophor, cotton swab, electric clipper, surgical board, 4 rubber bands, scissors, 2 large ophthalmic curved forceps, 1 needle holder, hemostatic forceps 1 piece, 6-0 suture with needle, 3-0 suture, suture needle, electronic scale, ventilator;

[0026] S2. Select rats in good condition, male, 220-250g, weigh them, lie supine on the surgical board with a rubber band, shave the left side of the sternum, and wipe the shaved area with iodine for disinfection. Cut the skin 4 cm longitudinally on the left side of the sternum, bluntly separate the subcutaneous muscle, and expose the pleura between the third and fourth ribs;

[0027] S3. Cut the skin 4 cm longitudinally on the left side of the sternum, bluntly separate the subcutaneo...

Embodiment 2

[0034] A preparation method of rat myocardial ischemia-reperfusion model, comprising the following steps:

[0035] S1. Surgical equipment needed for the preparation of the experiment: 10% water and 1ml syringe, iodophor, cotton swab, electric clipper, surgical board, 4 rubber bands, scissors, 2 large ophthalmic curved forceps, 1 needle holder, hemostatic forceps 1 piece, 6-0 suture with needle, 3-0 suture, suture needle, electronic scale, ventilator;

[0036] S2. Select rats in good condition, male, 220-250g, weigh them, lie supine on the surgical board with a rubber band, shave the left side of the sternum, and wipe the shaved area with iodine for disinfection. Cut the skin 4 cm longitudinally on the left side of the sternum, bluntly separate the subcutaneous muscle, and expose the pleura between the third and fourth ribs;

[0037] S3. Cut the skin 4 cm longitudinally on the left side of the sternum, bluntly separate the subcutaneous muscles, expose the pleura between the th...

experiment example

[0044] Four groups of rats were taken, and the number of rats in each group was 5, one group was fed normally, and the other four groups were operated according to the above-mentioned embodiment, and finally prepared a comparative electrocardiogram model and the infarct area of ​​the rat heart, wherein the experimental group The heart rate is low and the infarct size is large.

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PUM

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Abstract

The invention discloses a preparation method of a rat myocardial ischemia reperfusion model. The preparation method comprises the following steps of: S1, preparing surgical instruments required by anexperiment as follows: 10% water, a 1ml injector, iodophor, a cotton swab, an electric clipper, a surgical plate, four rubber bands, scissors, two large ophthalmic bent forceps, a needle holder, hemostatic forceps, a 6-0 needle suture line, a 3-0 suture line, a suture needle, an electronic scale and a respirator; and S2, selecting a rat which is in a relatively good state, is male and has the weight of 220-250 g, weighing the rat, anesthetizing the rat, lying the rat on the back, fixing the rat on the surgical plate by using the rubber bands, shaving the left side of the sternum, wiping the iodophor on the shaved part for disinfection, longitudinally cutting the skin on the left side of the sternum by 4 cm, bluntly separating subcutaneous muscles, and exposing the pleura between the separated third and fourth ribs. Compared with an existing preparation method of a mouse myocardial ischemia reperfusion model, the preparation method of the rat myocardial ischemia reperfusion model has the advantages that the control is simple, the survival time of the rat is long, and the perfusion effect is good.

Description

technical field [0001] The invention relates to the technical field of rat myocardial perfusion models, in particular to a method for preparing a rat myocardial ischemia-reperfusion model. Background technique [0002] Existing mouse myocardial ischemia / reperfusion (MIR) models At present, the preparation of mouse myocardial ischemia models mostly adopts drug induction methods, such as vasopressin (pituitrine) isoproterenol, etc., these drugs induce The role of the complex, and can not accurately achieve reperfusion. [0003] Guo et al. prepared a mouse myocardial ischemia / reperfusion model through surgery in 1998. They reported that the reperfusion time could reach 12h. However, this method has high requirements for experimental equipment and strict requirements for maintenance conditions for long-term reperfusion, and this method is inefficient, and one experimenter can do at most two per day. In view of the disadvantages of the above methods, the researchers have develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00A61D3/00
CPCA61D1/00A61D3/00A61D7/00
Inventor 闫奎坡朱翠玲孙彦琴朱明军徐亚洲刘刚
Owner THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF TCM
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