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Colorectal cancer related estrogen plasma metabolism small molecule screening method and application thereof

A technology for metabolizing small molecules and colorectal cancer, which is applied in the field of screening small molecules for colorectal cancer-related estrogen plasma metabolism, achieving low detection limits, good experimental basis, and high-throughput detection limits

Active Publication Date: 2020-11-06
NANJING MEDICAL UNIV
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Problems solved by technology

In addition, in the current technical means, there is no biomarker for colorectal cancer-related estrogen metabolism changes based on the difference in the incidence of colorectal cancer between men and women

Method used

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  • Colorectal cancer related estrogen plasma metabolism small molecule screening method and application thereof
  • Colorectal cancer related estrogen plasma metabolism small molecule screening method and application thereof
  • Colorectal cancer related estrogen plasma metabolism small molecule screening method and application thereof

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Experimental program
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Embodiment

[0041] 1. Determine the research object and group:

[0042] A total of 191 cases of colorectal cancer and 203 normal controls were included, all of whom were Han males; the cases were from September 2010 to the present, from the Affiliated Hospital of Nanjing Medical University, and all cases were confirmed by histopathology; the control group was selected from the same period Healthy people who underwent physical examination in the hospital and no tumor was detected; case and control groups were frequency matched according to age (±5 years old); demographics (including age, smoking, drinking, etc.) and clinical information ( (including tumor location, tumor grade, clinical stage, etc.) were obtained by professionally trained personnel through a standardized questionnaire; all subjects signed informed consent and 5mL of venous blood was collected, and plasma was separated after static centrifugation; this time The study was approved by the Ethics Committee of Nanjing Medical U...

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Abstract

The invention discloses a colorectal cancer related estrogen plasma metabolism small molecule screening method and application thereof. The method comprises the following steps: determining research objects and groups; performing metabonomics analysis by ultra-high performance liquid chromatography-tandem mass spectrometry, and screening colorectal cancer related estrogen metabolism small molecular markers; and carrying out methodology verification, data statistical analysis and biomarker screening. According to the invention, an efficient, accurate, high-flux and low-detection-limit UPLC-MS / MS technology is utilized; estrogen metabonomics analysis is performed on plasma of colorectal cancer patients and healthy control; an estrogen metabolism small molecular marker for early diagnosis ofcolorectal cancer is found, and the result is of great significance to clarification of the change rule of the plasma estrogen metabolite content of a patient with colorectal cancer and revelation ofthe effect of estrogen in the tumor occurrence and development process; by applying the screening method, the efficient colorectal cancer early diagnosis biomarker can be obtained, and a good experimental basis is provided for clinical diagnosis of colorectal cancer.

Description

technical field [0001] The invention relates to the technical field of colorectal cancer, in particular to a small molecule screening method and application of colorectal cancer-related estrogen plasma metabolism. Background technique [0002] Colorectal cancer is one of the most common malignant tumors. In China, the morbidity and mortality of colorectal cancer rank fifth among all tumors. The current epidemiological survey found that colorectal cancer is more common in middle-aged men, and the incidence rate of men is significantly higher than that of women; and female patients respond better to adjuvant chemotherapy than male patients. These studies suggest that sex hormones may be an important factor affecting the occurrence and development of colorectal cancer, and their in vivo levels may have clinical application value as biomarkers for early diagnosis of colorectal cancer. [0003] At present, the diagnostic methods of colorectal cancer mainly rely on imaging exami...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8675G01N30/8696Y02A90/10
Inventor 王美林杜牧龙顾冬英张正东黎书炜储海燕陈烨桦贲帅
Owner NANJING MEDICAL UNIV
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