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Small tissue block dehydration method

A technology for small tissues and tissue blocks, which is applied in sampling, instruments, measuring devices, etc., can solve the problems of tissue becoming brittle, increasing scientific research time and material costs, and difficulty in forming slices, so as to reduce reagent costs, increase success rate and experiment Efficiency, Effectiveness, Reliable Effects

Pending Publication Date: 2020-11-06
NANTONG UNIVERSITY
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Problems solved by technology

[0003] The technical problem to be solved: In view of the excessive dehydration existing in the prior art, the tissue becomes brittle and difficult to form when slicing, and the solution of tissue dehydration using gradient ethanol cannot be widely used for animal tissues / organs from small to large volumes, especially There are great difficulties in dehydration optimization of small pieces of tissue, and it is not easy to achieve the ideal dehydration effect; the number of ethanol tanks is large, and it needs to be replenished or replaced frequently because it is volatile. The above shortcomings will cause technical problems such as increased research time and material costs. The object of the invention is to provide a method for dehydrating small tissue pieces

Method used

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  • Small tissue block dehydration method

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Embodiment 1

[0028] A method for dehydrating a small tissue block, the steps are:

[0029] The first step is to configure IMS: industrial methylated ethanol Industrial Methylated Spirits, IMS, is made by adding 95% ethanol to 5% methanol and then undergoing methylation denaturation;

[0030] The second step, dehydration of small pieces of tissue: 1 / 8 of the kidney on the same side of the same mouse, 99% IMS channel I, 99% IMS channel II, 99% IMS channel III, immerse the tissue for 1 h in each channel;

[0031] The third step: tissue transparent in xylene, dipped in wax, embedded, sliced, baked, baked, and stained with hematoxylin and eosin.

[0032] The specific steps of the xylene tissue transparency, wax immersion, embedding, sectioning, baking, baking and hematoxylin and eosin staining are as follows:

[0033] The specific steps for xylene tissue transparency are: xylene I course 15 minutes, xylene II course 15 minutes.

[0034] The specific steps of wax immersion are: paraffin wax I ...

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Abstract

The invention discloses a small tissue block dehydration method which is applied to an animal tissue paraffin block preparation process. The method comprises the following steps: adding 5% methanol into 95% ethanol, carrying out methylation denaturation to prepare IMS; forming a 99% IMS channel I, a 99% IMS channel II and a 99% IMS channel III, and immersing tissues in each channel for 1 hour; andperforming xylene tissue transparent treatment, wax dipping, embedding, slicing, slice baking, slice baking and hematoxylin (dyed cell nucleus being blue) eosin (dyed cell pulp being pink) dyeing. Adehydration reagent is improved, the number of cylinders is reduced, the scientific research cost is reduced, and small tissues are dehydrated to achieve a more stable effect.

Description

technical field [0001] The invention belongs to the field of tissue block dehydration, and in particular relates to a small tissue block dehydration method. Background technique [0002] Existing reagents for tissue dehydration usually use graded ethanol, prepared by absolute ethanol and deionized water, from low concentration to high concentration: 80% ethanol I channel, 90% ethanol I channel, 95% ethanol I channel, 95% ethanol course II, 100% ethanol course I, 100% ethanol course II. Tissues were dehydrated by immersion in each reagent for 1 hour. But for small pieces of tissue, such a dehydration procedure usually leads to excessive dehydration, making the tissue brittle and difficult to shape when slicing. Therefore, most scholars will adjust by reducing the ethanol tank and / or reducing the immersion time of the tissue, but it is time-consuming and laborious, and it is difficult to obtain the desired effect. Contents of the invention [0003] The technical problem t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/44G01N1/36G01N1/28
CPCG01N1/30G01N1/36G01N1/44G01N1/286G01N2001/2873
Inventor 吴圆圆
Owner NANTONG UNIVERSITY
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