Cell multi-parameter detection micro-nano sensor and manufacturing method thereof

Abstract

The invention provides a cell multi-parameter detection micro-nano sensor and a manufacturing method thereof. The sensor comprises a glass substrate (1) and a PDMS sheet (2) which are attached to eachother; a flow groove (7) is formed in the binding surface of the PDMS sheet (2); a flow channel is formed on the glass substrate (1) after lamination; a sample inlet hole (5) and a sample outlet hole(6) are formed in two ends of the flow groove (7); an open box dam for cells to flow in and remain is arranged in the flow groove (7), an opening of the flow groove (7) faces the sample injection direction, a plurality of micro electrode blocks are arranged at the positions, corresponding to the open box dam, of the binding surface of the glass substrate (1) and located below the open box dam, and a height gap smaller than the outer diameter of a cell individual to be detected is reserved between the open box dam and the glass substrate (1); each micro electrode block is provided with an outgoing line (4) made of a Pt materials. According to the invention, a plurality of parameters of cells can be detected, and online continuous monitoring can be realized.

Description

technical field [0001] The invention relates to the technical field of cell detection and micro-nano sensors, in particular to a cell multi-parameter detection micro-nano sensor and a manufacturing method thereof. Background technique [0002] In the field of biology, parameters such as temperature, conductivity, and pH value are indispensable and important indicators for studying the life process of organisms in the field of life sciences. In life science research, on the one hand, various physiological and chemical reactions are constantly going on in living cells, and the performance closely related to these reactions is the change of temperature, conductivity, and pH value. On the other hand, in the case of external stimuli, cells will quickly adjust their metabolic activities in response to environmental changes, which will also be accompanied by significant changes in parameters such as temperature, conductivity, and pH. Therefore, the realization of real-time detecti...

AI Technical Summary

Problems solved by technology

[0005] The above microfluidic devices applied to the detection of single-cell parameters still have the following problems: 1) Only the detection of a single parameter can be realized, but the integrated simultaneous detection and real-time online continuous monitoring of multiple parameters cannot be realized

2) A chip that can realize the integration of cell detection has a complex structure and requires external sampling or control equipment

3) The chip with a simple structure only realizes single cell capture and lysis, and does not integrate all detection steps

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