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Dry homocysteine test card and application thereof

A homocysteine ​​and test card technology, applied in the field of dry homocysteine ​​test cards, can solve the problems of poor clinical sensitivity

Pending Publication Date: 2020-10-30
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two methods are easy to operate and simple in structure, but there is a major defect that most of the homocysteine ​​in the blood binds to proteins through disulfide bonds to form oxidized homocysteine, which is not used in the above application documents Reducing agents reduce oxidized homocysteine, so they can only detect free reduced homocysteine ​​in blood samples, which has poor clinical sensitivity

Method used

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  • Dry homocysteine test card and application thereof
  • Dry homocysteine test card and application thereof
  • Dry homocysteine test card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment provides a preparation method of the dissociative agent R1 of the dry-type homocysteine ​​detection reagent, which is prepared according to the following method.

[0033] Dissociating agent R1-1: Measure 1L of purified water, add the following substances respectively and mix well: 0.5g sodium chloride, 6g tris, 0.015g β-mercaptoethanol, 0.15g serine, adjust the pH with hydrochloric acid value to 8.0.

[0034] Dissociating agent R1-2: Measure 1L of purified water, add the following substances respectively and mix well: 0.5g sodium chloride, 6g trishydroxymethylaminomethane, 0.03g dithiothreitol, 0.15g serine, use hydrochloric acid Adjust the pH to 8.0.

[0035] Dissociating agent R1-3: Measure 1L of purified water, add the following substances respectively and mix well: 0.5g sodium chloride, 6g trishydroxymethylaminomethane, 0.057g tris(2-carboxyethyl)phosphine, 0.15g Serine, adjust the pH to 8.0 with hydrochloric acid.

[0036] Dissociating agent R1-4...

Embodiment 2

[0038] This embodiment provides a method for preparing a dry-type homocysteine ​​detection test paper, which is prepared according to the following method.

[0039] (1) Preparation of enzyme pad 3:

[0040] Enzyme pad treatment liquid preparation: Measure 1L of purified water, add the following substances respectively and mix well: 0.5g sodium chloride, 2.4g tris, 1g Tween 20, 0.4g magnesium chloride, 30g trehalose, 10g Polyvinyl alcohol 10, 300KU peroxidase, 500U pyruvate oxidase, adjust the pH value to 8.0 with hydrochloric acid.

[0041] Enzyme pad 3 treatment: Cut the tea filter paper (Shanghai Kaining Paper: RF17) into 5mm×300mm, soak it in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, and place it horizontally in an oven at 45°C to dry for 1 hour, take it out and set aside.

[0042] (2) Preparation of enzyme cycle reaction layer 5:

[0043] Preparation of enzyme cycle reaction layer treatment solution: Measure 1L of purified wate...

Embodiment 3

[0053] This embodiment provides a method for preparing a dry-type homocysteine ​​detection test paper, which is prepared according to the following method.

[0054] (1) Preparation of enzyme pad 3:

[0055] Enzyme pad treatment solution preparation: Measure 1L of purified water, add the following substances respectively and mix well: 0.5g sodium chloride, 2.4g tris, 1g Tween 20, 0.4g magnesium chloride, 30g sucrose, 10g poly Vinyl alcohol 10, 300KU peroxidase, 500U pyruvate oxidase, adjust the pH value to 8.0 with hydrochloric acid.

[0056] Enzyme pad 3 treatment: Cut the tea filter paper (Shanghai Kaining Paper: RF17) into 5mm×300mm, soak it in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, and place it horizontally in an oven at 45°C to dry for 1 hour, take it out and set aside.

[0057] (2) Preparation of enzyme cycle reaction layer 5:

[0058] Preparation of enzyme cycle reaction layer treatment solution: Measure 1L of purified wat...

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Abstract

The invention relates to a dry homocysteine test card and application thereof, the dry homocysteine test card comprises a dissociation liquid R1 and dry chemical test paper, the dissociation liquid R1comprises a reducing agent, serine and a buffer solution; the dry chemical test paper comprises a supporting layer, a chromatographic membrane, an enzyme pad, a blood filtering layer, an enzyme circulation reaction layer, a diffusion layer and a plastic sheet; the chromatographic membrane, the enzyme pad, the blood filtering layer, the enzyme circulation reaction layer and the diffusion layer aresequentially overlapped on the supporting layer from bottom to top, the length of the chromatographic membrane is greater than that of the enzyme pad, the blood filtering layer, the enzyme circulation reaction layer and the diffusion layer, and the chromatographic membrane is fixed at one end close to the supporting layer and extends towards the other end of the supporting layer; a plastic sheetis arranged near one end, far away from the enzyme pad, of the supporting layer, a color developing region is arranged on the plastic sheet, the plastic sheet is positioned above the chromatographic membrane and is parallel to the chromatographic membrane, and the color developing region covers partial region of the chromatographic membrane. The test card has the advantages of being capable of detecting all homocysteine in human blood and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a dry-type homocysteine ​​test card and its application. Background technique [0002] Homocysteine ​​(HCY) is an amino acid containing sulfur molecules in the blood, which is converted from methionine. It is produced by demethylation of methionine in vivo, and is mainly metabolized through remethylation and transsulfuration pathways. Need methionine synthase, cystathionine β synthase (CBS) and vitamin B12, folic acid, vitamin B6 to participate. Elevated homocysteine ​​can be caused by enzyme dysfunction or vitamin deficiency. Under normal circumstances, HCY will be rapidly converted into glutathione and S-adenosylmethionine. These are two very important substances in the living body, and are closely related to liver detoxification, the removal of free radicals in the body, and repair of DNA. The increase of HCY means that the lack of these two substances will...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 邹继华刘献文薛杨波王幸媛贾江花方亮
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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