Screening reagent for detecting core molecules of DNA damage signal path and application of screening reagent
A DNA damage and signaling pathway technology, applied in the field of molecular biology, can solve problems such as premature aging, increased cancer tendency, immune deficiency, etc.
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[0294] The present invention is illustrated by human liver cancer Huh7 and liver cancer cell Huh7 treated with SR-4835, but the present invention is not limited to these two types of cells, and the detection reagent of the present invention can be applied to liver cancer cell lines including SMMC7721, HCCLM3, etc. It can detect DNA damage signals in clinical liver cancer tissues and paracancerous tissues such as lung cancer, gastric cancer, pancreatic cancer, ovarian cancer and other tumors.
[0295] In the present invention, the human liver cancer cell line Huh7 is cultivated, and then treated with SR-4835 (100nM) for 24 hours, and the change of core molecules in the treated cell line is detected by the screening reagent of the DNA damage signaling pathway of the present invention.
[0296] The human liver cancer Huh7 used in the present invention was purchased from ATCC Company of the United States, the Shanghai Cell Bank, DMEM medium, and fetal bovine serum were purchased fr...
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