Screening reagent for detecting core molecules of DNA damage signal path and application of screening reagent

Abstract

The invention provides a screening reagent for detecting core molecules of DNA damage signal path and an application of the screening reagent. The PCR reagent comprises PCR reaction primers for detecting ABL1 genes, APEX1 genes, ATM genes, ATR genes and other related genes, reference genes and the like. According to the invention, core molecules related to a DNA damage signal path are concentratedon a flat plate; a real-time fluorescent quantitative PCR reaction is carried out once; core molecules for regulating and controlling DNA damage signal pathways in cells are reflected, a drug-treatedliver cancer Huh7 and an untreated liver cancer Huh7 are compared for example, possible pathways of the core signal molecules causing DNA damage are discussed, and most direct evidences are providedfor researching regulation and control of key proteins; according to the invention, the core signal molecule of DNA damage is rapidly and accurately found from the transcription level, and a powerfultool is provided for mechanism discussion of new targeting drugs, development of targeting tumor inhibitors and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a screening reagent for detecting core molecules of a DNA damage signaling pathway and an application thereof. Background technique [0002] Every cell of our body produces thousands of DNA lesions every day due to various internal and environmental factors. These lesions, if not properly repaired, can lead to mutations and broader genomic aberrations that threaten cell viability and ultimately lead to cancer. DNA lesions will trigger a series of well-organized and multifaceted synergistic responses inside the cell to jointly combat this genotoxic stress, to detect DNA damage, indicate its existence, and mediate its repair. This series of defense responses That is called DNA damage response (DNA damage response, DDR). DNA repair is critical in many biological processes, including the development of the immune system, meiotic recombination, and the prevention of a va...

AI Technical Summary

Problems solved by technology

Inherited DNA repair defects can be catastrophic, leading to developmental abnormalities, increased predisposition to cancer, immune deficiencies, neurodegenerative diseases and premature aging

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