Degradation process of akebia trifoliata peel pectin

A technology of Akebia clover and peel, applied in the biological field, can solve the problems of low degradation efficiency, low yield of oligosaccharides, large molecular weight, etc., and achieve the effects of precise degradation, mass production, and change of bubble content and thermal conductivity

Pending Publication Date: 2020-10-30
TONGREN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex structure of pectin and its large molecular weight, the microenvironment in the molecule affects the contact between pectinase and the action site, so there are certain limitations in directly degrading pectin with pectinase, the degradation efficiency is low, and the yield of oligosaccharides is low. Low, difficult to select suitable enzymes to efficiently prepare pectin oligosaccharides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Degradation process of akebia trifoliata peel pectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A degradation process of Akebia trifoliate peel pectin, comprising the steps of:

[0032] (1) Hydrolysis treatment:

[0033] Put the peel pectin into the acid solution for acid hydrolysis treatment, the hydrolysis time is controlled to 5h, and the acid hydrolyzate is obtained after completion; the acid solution is a phosphoric acid solution with a concentration of 1mol / L, and the peel pectin is put into The weight ratio of the two corresponding to the acid solution is 1:95; the temperature of the acid solution is always kept at 70°C during the acid hydrolysis treatment;

[0034] (2) Ventilation treatment:

[0035] Into the acid hydrolyzate of step (1) gained, pass into compound gas, the duration of control feeding is 6h; Described compound gas is to be formed by mixing oxygen and argon correspondingly according to the volume ratio of 2:1;

[0036] (3) Ultrasonic treatment:

[0037] Submerge the probe of the ultrasonic instrument in the acid hydrolyzate after step (2)...

Embodiment 2

[0043] A degradation process of Akebia trifoliate peel pectin, comprising the steps of:

[0044] (1) Hydrolysis treatment:

[0045] Put the peel pectin into the acid solution for acid hydrolysis treatment, the hydrolysis time is controlled to 5.5h, and the acid hydrolyzate is obtained after completion; the acid solution is a phosphoric acid solution with a concentration of 1.3mol / L, and the peel pectin When put into the acid solution, the corresponding weight ratio of the two is 1:98; during the acid hydrolysis treatment, the temperature of the acid solution is always kept at 74°C;

[0046] (2) Ventilation treatment:

[0047] Pass into compound gas in the acid hydrolyzate of step (1) gained, the duration of control feeding is 7h; Described compound gas is to be formed by mixing oxygen and argon correspondingly according to volume ratio 2.6:1;

[0048] (3) Ultrasonic treatment:

[0049] Submerge the probe of the ultrasonic instrument in the acid hydrolyzed solution after ste...

Embodiment 3

[0055] A degradation process of Akebia trifoliate peel pectin, comprising the steps of:

[0056] (1) Hydrolysis treatment:

[0057] Put the peel pectin into the acid solution for acid hydrolysis treatment, the hydrolysis time is controlled to 6h, and the acid hydrolyzate is obtained after completion; the acid solution is a phosphoric acid solution with a concentration of 1.5mol / L, and the peel pectin is put into The weight ratio of the two corresponding to the acid solution is 1:100; the temperature of the acid solution is always kept at 75°C during the acid hydrolysis treatment;

[0058] (2) Ventilation treatment:

[0059] In the acid hydrolyzate obtained in step (1), pass into composite gas, and the time length of control feeding is 8h; Described composite gas is to be formed by mixing oxygen and argon correspondingly according to the volume ratio of 3:1;

[0060] (3) Ultrasonic treatment:

[0061] Submerge the probe of the ultrasonic instrument in the acid hydrolyzed sol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a degradation process of akebia trifoliate peel pectin, which belongs to the technical field of biology and comprises the following steps: (1) hydrolysis treatment, (2) aeration treatment, (3) ultrasonic treatment, (4) centrifugal treatment and (5) enzymolysis treatment. The method disclosed by the invention is simple in overall process, is beneficial to popularization andapplication, can be used for efficiently preparing a large amount of oligogalacturonic acid, and lays a foundation for subsequent separation of oligogalacturonic acid pure products, and identificationof activity and structures of the oligogalacturonic acid pure products.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a degradation process of akebia trifoliate peel pectin. Background technique [0002] Akebia trifoliata (scientific name: Akebia trifoliata (Thunb.) Koidz.) belongs to the Akebia family, and Akebia belongs to deciduous woody vines. Stem bark grayish brown, palmate compound leaves alternate or clustered on short branches; petiole straight, leaf blade papery or thin leathery, ovate to broadly ovate, apex usually blunt or slightly concave, base truncated or rounded , with wavy teeth or shallow lobes on the edge, dark green above and light green below; racemes emerge from the clustered leaves on short branches, the total pedicel is slender, male flowers: pedicels are silky, lavender, broadly oval or oval , filaments are very short, and the drug chamber is curved inward during flowering; degenerated carpels are oblong-conical. Female flowers: pedicel slightly thicker than ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/00C12P19/04
CPCC12P19/00C12P19/04
Inventor 张孟琴徐路鲁道旺张迅
Owner TONGREN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap