Water improvement micro-ecological preparation
A micro-ecological preparation and water body improvement technology, applied in the direction of microorganisms, microorganism-based methods, biological water/sewage treatment, etc., can solve problems such as water quality deterioration, fish death, fishery and aquaculture hazards, etc.
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preparation example Construction
[0024] (2) Preparation of seed solution
[0025] According to the ratio (volume percentage) of 0.05%-0.5%, add the inoculum prepared in step (1) in the 250ml Erlenmeyer flask containing beef extract peptone liquid culture, 30 ℃, 200 rpm / min shaker culture 21-24h, obtain Bacterial liquid containing Bacillus subtilis at a concentration not less than 5×10 9 CFU / ml. The seed solution preparation method of Rhodopseudomonas palustris and Brevibacillus laterosporus is the same as that of Bacillus subtilis. Saccharomyces cerevisiae (Sacchuromyces cerevisiue) was added to the culture medium containing YPD for 48-72h, and the rest of the steps were the same as above;
[0026] (3) Fermentation culture
[0027] A: The bacterial seed solution obtained in step (2) is inserted into the broth medium according to the ratio of 1-10% (volume percentage), and then cultivated at 28-34°C and the speed of seeding bed is 170-210rpm / min 22-25 hours to obtain individual strain preparations;
[002...
specific Embodiment approach 2
[0031] (1) Preservation and activation of strains
[0032] Bacillus subtilis was stored at 4°C in TSB medium containing 15% glycerol. The formula of TSB medium: Weigh 30g of Tryptic Soy Broth (TSB) medium powder and add it to 1000ml of distilled water, mix well. Ready to use after autoclaving. Use a micropipette to pipette 10μl of the bacterial solution into 3-5ml of liquid beef extract peptone medium, pH ≈ 7.0-7.2; culture on a shaker at 200rmp / min, 30°C, 18-20 hours. The preservation and cultivation methods of Rhodopseudomonas palustris and Brevibacillus laterosporus are the same as those of Bacillus subtilis;
[0033] Saccharomyces cerevisiae was maintained at 4°C in YPD medium containing 15% glycerol. The formula of YPD substratum: take by weighing 49g YcastPeptoncDextrosc Agar (YPD) substratum powder and add 1000ml distilled water, mix, after high-pressure fire bacteria, for subsequent use;
[0034] (2) Preparation of seed solution
[0035] According to the ratio (vo...
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