Composite microbial agent with biocontrol function and preparation method and application thereof

A compound microbial agent and functional technology, which is applied in the preparation of organic fertilizers, microbial treatment, combined biological treatment, etc., can solve the problems of low survival rate of probiotics, inconvenient agricultural production, complicated preparation process, etc.

Inactive Publication Date: 2020-10-30
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there are many reports on the biocontrol application of soil-borne diseases. For example, Chinese patent CN201510769449.9 discloses a method for growing bio-organic fertilizers using pig manure and mushroom dregs. Bacillus subtilis is selected to decompose pig manure and mushroom dregs, and then Compounding probiotics that can antagonize or inhibit harmful fungi, the survival rate of probiotics in this method is low, and a large number of probiotics must be used to ensure the survival rate, which increases the cost; Chinese patent CN201410514541.6 discloses a method that utilizes microbial fermentation A process for preparing pig manure bio-organic fertilizer by adding 10% to 20% seaweed in the composting ingredients, so that the prepared bio-organic fertilizer has the advantages of seaweed fertilizer, but the preparation process is complicated and inconvenient for practical agricultural production; Chinese patent CN201010228347.3, CN201310666396.9, and CN20130308892.7 all provide straw decomposing bacterial agents for composting fermentation, but straw composting fermentation has the disadvantages of laborious and high cost

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  • Composite microbial agent with biocontrol function and preparation method and application thereof
  • Composite microbial agent with biocontrol function and preparation method and application thereof
  • Composite microbial agent with biocontrol function and preparation method and application thereof

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preparation example Construction

[0039] The present invention also provides the preparation method of the composite microbial bacterial agent with biocontrol function described in the above technical scheme, comprising the following steps:

[0040] Mix pig manure, mushroom dregs and straw to obtain a premix;

[0041] Beauveria bassiana is mixed with the premix to carry out the first fermentation to obtain the first fermented material;

[0042] Trichoderma harzianum is mixed with the premix, and the second fermentation is carried out to obtain the second fermented material;

[0043] Mixing the first fermented material with the second fermented material to obtain the primary fermented material;

[0044] Pseudomonas fluorescens is mixed with the primary fermentation material, and the third fermentation is carried out to obtain the composite microbial bacterial agent with biocontrol function.

[0045]In the present invention, the water content is preferably controlled to be 20%-45% during the first fermentation...

Embodiment 1

[0059] 1. Mixing of raw materials: pig manure, mushroom dregs, and straw are prepared according to the mass ratio of 40:30:25, stacked in the fermentation tank, and mixed evenly;

[0060] 2. Sprinkle the prepared Beauveria bassiana Sanju-22, the biological preservation number is CGMCCNo.17974, and Trichoderma harzianum fermented liquid with 5%, 20% inoculum respectively into the above-mentioned mixed raw materials, well mixed.

[0061] 3. Control the water content to 20%, and carry out primary fermentation respectively. Keep moist during the fermentation process, regularly observe the changes in water content, and replenish water in time. When the temperature of the pile reaches above 55°C, turn it over with a turning machine. The initial fermentation time is 15 days. After fermentation, the two fermented products are mixed to obtain the primary fermentation material.

[0062] 4. Sprinkle the Pseudomonas fluorescens fermentation liquid obtained by separate fermentation evenl...

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Abstract

The invention provides a composite microbial agent with a biocontrol function and a preparation method and application of the composite microbial agent, and belongs to the technical field of bio-organic fertilizers. By optimizing the proportion, the multifunctional effect of the compound microbial agent is fully exerted, antagonism does not exist between the compound microbial agent and the compound microbial agent, the effective viable count reaches the maximum, and the compound microbial agent has the effects of generating active substances for promoting crop growth, supplying fertilizer tocrops and leading and planting beneficial microorganism dominant groups to crop root system soil, meanwhile, the trichoderma harzianum in the microbial agent is a biocontrol bacterium which is commonly used for preventing plant diseases caused by pathogenic bacteria such as pythium, rhizoctonia solani, fusarium, rhizopus nigricans, cylindrosporium, sclerotinia sclerotiorum, sclerotium rolfsii andthe like, and can effectively prevent soil-borne diseases of crops.

Description

technical field [0001] The invention relates to the technical field of bio-organic fertilizers, in particular to a compound microbial agent with bio-control function and its preparation method and application. Background technique [0002] my country is a large agricultural country. During the entire phenological period of crops, pests and diseases of crops are important factors affecting their yield. important. However, my country's agriculture mainly uses chemical pesticides and chemical fungicides to prevent and control pests and diseases. Although chemical pesticides can effectively prevent and control pests and diseases, the long-term over-reliance on chemical pesticides results in changes in farmland soil quality, decline in fertility, residues of crop products, decline in quality, and food and environmental pollution. . In order to reduce the use of chemical pesticides, there is a trend in the world to develop biopesticides or microbial fertilizers with biocontrol pro...

Claims

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Application Information

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IPC IPC(8): C05F15/00C05F17/10C05F17/20C05F17/50
CPCC05F3/00C05F17/10C05F17/20C05F17/50C05F5/00C05F11/00C05F11/08Y02W30/40
Inventor 李肖宇郭小红刘玉珍翟修彩危月辉揭云东李英武陈晓燕骆毛喜
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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