Preparation method of tissue engineered bone with regeneration ability

Abstract

The invention discloses a preparation method of tissue engineered bone with a regeneration ability. The preparation method comprises the following steps of S1, preparation of collagen matrix, S2, preparation of mesenchymal stem cells, S3, preparation of bone marrow, S4, cultivation and preparation of semi-finished tissue engineered bone and S5, cultivation and preparation of the tissue engineeredbone with the regeneration ability. The method is short in osteogenic induction time, high in safety, low in cost, and suitable for simple bone tissue production, the prepared tissue engineered bone can be completely fused with body tissues after being embedded in a body and then grow into a part of the body, so that a purpose of tissue regeneration is achieved, the problem that fixed tissue materials cannot be synchronized with the growth and degeneration of the body is completely solved, and the tissue engineered bone can further be shaped, spliced and matured in the body and is an ideal regenerative medicine final product and material product.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a method for preparing tissue-engineered bone with regenerative capacity. Background technique [0002] Due to certain factors such as trauma, infection, tumor resection or congenital diseases, the bone loses some bone mass and forms a large gap, which is called bone defect. Bone defects are relatively common clinically, and their treatment has always been a difficult point in clinical treatment. Bone transplantation provides the possibility to solve the above problems. At present, autologous bone is still the first choice of bone graft source, but its supply is limited, and it is prone to the dilemma of "removing the east wall to fill the west wall" in application, which is difficult to meet the clinical needs. Therefore, finding a suitable bone graft substitute has become the primary problem in the research and repair of bone defects. [0003] Tissue engineering bon...

AI Technical Summary

Problems solved by technology

With the development of technology, absorbable and tissue-compatible materials are also used in the treatment of bone defects, but at present, it is difficult for tissue engineering materials and tissue-engineered bone to form blood vessels, and to form a balanced state of bone formation and resorption, and regenerate tissue There are still great differences in structure and function from normal tissues, and it is difficult to complete angiogenesis and bone marrow reconstruction in the treatment of bone loss, and cannot completely replace the missing function