Method for capturing whole genome from adenovirus, primer group and kit

Abstract

The invention relates to a method for capturing a whole genome from adenovirus, a primer group and a kit. According to the method, the primer group and the kit, the whole genome of the adenovirus canbe conveniently and rapidly amplified by a small amount of RNAs, and a sequence of the whole genome of the adenovirus can be obtained by directly docking a secondary sequencing library-building reagent and a tertiary sequencing platform.

Description

technical field [0001] The invention belongs to the field of virus detection, and in particular relates to a method for capturing the whole genome of adenovirus, a primer set and a kit. Background technique [0002] Human adenovirus (HAdV) pneumonia is one of the more serious types of community-acquired pneumonia in children. It mostly occurs in children aged 6 months to 5 years. Some children have severe clinical manifestations and many pneumonia complications. Severe cases are prone to Residual slow-moving airway and lung diseases are one of the important causes of death and disability in infants and young children. [0003] HAdV belongs to the genus of mammalian adenovirus, which is a non-enveloped double-stranded DNA virus. At least 90 genotypes have been found, which are divided into 7 subgenuses A-G. The tissue infectivity and pathogenicity of different types of HAdV, The characteristics such as endemic area are different. The HAdVs associated with respiratory tract ...

AI Technical Summary

Problems solved by technology

However, the differences in application scenarios and many complex factors jointly affect the success rate of obtaining the whole genome of pathogens. At the same time, there are also problems such as time, manpower, and high sequencing costs for front-line personnel under the heavy task of testing.

Therefore, pathogenic metagenomic sequencing (mNGS) can be used for the diagnosis of negative samples, but it is not easy to obtain the whole genome information, and there are reasons such as complicated operation and relatively long detection time (requiring 24-72 hours). In the detection of adenovirus The purpose of large-scale promotion and rapid diagnosis cannot be achieved

As a technical supplement, the genome capture method based on amplification is a "culture-free" virus genome-specific enrichment sequencing solution, which is easy to operate and low in cost, and is suitable for the current needs of epidemic prevention and control. Some probe capture sequencing methods have complex operation steps, mainly including 7 different technical operations, and each part of the operation requires more than 5 steps, and many can even reach 33 steps, and once the operation is performed, in order to meet the detection requirements , the operations within each part cannot be paused and must be completed continuously

This brings a lot of uncontrollability and operational complexity to the actual operation.

Images