Acupoint injection for improving immune function

A technology for immune function and injections, applied in the direction of medical raw materials derived from mammals, medical preparations containing active ingredients, drug delivery, etc., can solve difficult standardized preparation and quality control, cannot obtain sufficient quantity and purity of PBMC, and reduce PRP Purity and other issues

Pending Publication Date: 2020-10-23
杭州嘉耀医疗健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method does not adopt the standard preparation method of PBMC, cannot obtain sufficient quantity and purity of PBMC, reduces the purity of PRP at the same time, and it is difficult to carry out standardized preparation and quality control

Method used

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  • Acupoint injection for improving immune function
  • Acupoint injection for improving immune function
  • Acupoint injection for improving immune function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Platelet-rich plasma was mixed with peripheral blood mononuclear cells at a volume ratio of 1:1.

[0039] The preparation method of peripheral mononuclear cells (PBMC):

[0040] Take 30-50 ml of human or rat peripheral blood, anticoagulate with 3% sodium citrate, collect under sterile conditions, fully mix with the same amount of Hank's solution, slowly add to the lymphocyte separation medium, and centrifuge horizontally at 2000 r / min Centrifuge at high speed for 30 min, take the narrow band of white cloud layer at the interface between the upper and middle layers, and obtain the PBMCs liquid containing platelets. Absorb mononuclear cells, put them into another centrifuge tube, add more than 5 times the volume of Hank's solution, and rotate horizontally Centrifuge at 1500 r / min for 10 min, and wash the cells twice. Hypotonically lyse the red blood cells to obtain pure PBMCs liquid, and count under a microscope.

[0041] Platelet-rich plasma (PRP) is prepared by:

[00...

Embodiment 2

[0071] PBCM and PRP ratio: 2:1, the preparation method of PBCM and PRP is the same as embodiment 1.

[0072] mouse carbon clearance test

[0073] Method: Same as 1.1.

[0074] As shown in Table 5, compared with the blank control group, PBMC, PRP and PBMC+PRP showed an upward trend in the carbon clearance phagocytosis index, thymus coefficient / body weight ratio and spleen coefficient / body weight ratio of mice, and the differences were statistically significant. academic meaning ( P <0.01), the effect of PBMC+PRP group was better than that of PBMC and PRP group, but only the effect on spleen coefficient was significantly improved (P<0.05).

[0075] Table 5 Mouse carbon clearance results

[0076]

[0077] Note:** P # P <0.05 compared with PBMC or PRP group

[0078] 2.2 Phagocytosis of chicken red blood cells by mouse peritoneal macrophages

[0079] Method: Same as 1.2.

[0080] As shown in Table 6, compared with the blank control group, PBMC, PRP and PBMC+PRP can signi...

Embodiment 3

[0097] PBCM and PRP ratio: 1:2, the preparation method of PBCM and PRP is the same as embodiment 1.

[0098] mouse carbon clearance test

[0099] Method: Same as 1.1

[0100] As shown in Table 9, compared with the blank control group, PBMC, PRP and PBMC+PRP showed an upward trend in the carbon clearance phagocytosis index, thymus coefficient / body weight ratio and spleen coefficient / body weight ratio of mice. The thymus coefficient index was significantly better than that of the control group (P<0.01), and the effect of the PBMC+PRP group on the three indexes was better than that of the PBMC and PRP group, among which the spleen coefficient and thymus coefficient index had a significant effect (P<0.05) .

[0101] Table 9 Mouse carbon clearance results

[0102]

[0103] Note:** P # P <0.05 compared with PBMC or PRP group

[0104] 3.2 Phagocytosis of chicken red blood cells by mouse peritoneal macrophages

[0105] Method: Same as 1.2

[0106] As shown in Table 10, compar...

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Abstract

The invention discloses an acupoint injection for improving an immune function, and belongs to the technical field of pharmaceutical injections for injection and preparation methods of the injections.The acupoint injection comprises platelet-rich plasma and peripheral blood mononuclear cells. The volume ratio of the platelet-rich plasma to the peripheral blood mononuclear cells is (1-2):(1-2). According to the acupoint injection, a method of combining PBMC with PRP to improve immunity is adopted, so that the phagocytic percentage of cells is obviously increased, the activity of NK cells is obviously enhanced, and the acupoint injection has the capacity of inducing lymphocyte proliferation and transformation and can improve the immune function of an organism.

Description

technical field [0001] The invention belongs to the technical field of drug injection for injection and its preparation method, and in particular relates to an acupoint injection for improving immune function. Background technique [0002] Platelet-rich plasma (platelet-rich plasma, PRP) refers to the highly concentrated product obtained from whole blood after centrifugation and separation, and its platelet level is usually more than 4 to 5 times that of normal whole blood. PRP contains various growth factors such as platelet-derived growth factor, β-transforming growth factor, vascular endothelial growth factor, epidermal growth factor, insulin-like growth factor, and fibroblast growth factor. At present, clinical results have shown that injection of PRP can treat osteoarthritis, promote angiogenesis, and surgical repair. [0003] Peripheral blood mononuclear cells (PBMC), including lymphocytes, monocytes, and macrophages, are cleared from the blood when PRP is extracted. ...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K9/00A61P37/04A61K35/15
CPCA61K35/15A61K35/16A61K9/0019A61P37/04A61K2300/00
Inventor 林亦望陈光舜
Owner 杭州嘉耀医疗健康科技有限公司
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