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Method to improve tolerance of Neoseiulus barkeri under co-stress of high temperature and low humidity

A technology of Neoseius pasteurii, high temperature and low humidity, applied in animal feed, additional food elements, animal feed, etc., to avoid the cost of fitness and shorten the operation time

Inactive Publication Date: 2020-10-16
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of improving the stress resistance of predatory mites through short-term adversity exercise and feeding carbohydrates has not been reported yet.

Method used

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  • Method to improve tolerance of Neoseiulus barkeri under co-stress of high temperature and low humidity
  • Method to improve tolerance of Neoseiulus barkeri under co-stress of high temperature and low humidity
  • Method to improve tolerance of Neoseiulus barkeri under co-stress of high temperature and low humidity

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Experimental program
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Effect test

Embodiment 1

[0028] Breeding of Neoseius mite

[0029] For the specific feeding method of Neoseiius pasteurii, refer to the Chinese invention patent "Cultivation and identification method of a high-temperature-resistant strain of Neoseiius pastereii", the publication number of which is "CN109997791A".

[0030] The dehydration exercise of Neoseius pasterii includes the following steps:

[0031] (1) Put a round sponge slightly smaller than the petri dish in a petri dish with a diameter of 15 cm, and put a round black cloth with the same size as the sponge on the sponge; soak the sponge and black cloth with tap water completely Wet, the water surface is flush with the top of the sponge; put a circular plastic film on the black cloth, its diameter is slightly smaller than the black cloth but not less than 10 cm; place a petri dish with a diameter of 8 cm on the plastic film.

[0032] (2) Sieve the Neoseius mite reared in the fresh-keeping box through a 60-mesh sieve to obtain a mixture of "pr...

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Abstract

The invention provides a method to improve tolerance of Neoseiulus barkeri under co-stress of high temperature and low humidity, and the method includes: (1) feeding: using Aleuroglyphus ovatus feeding on wheat bran as prey of the Neoseiulus barkeri, and carrying out large-scale feeding and breeding under the following environmental conditions: temperature of 25 + / - 1 DEG C, relative humidity of 70-80% and photoperiod of 14 L: 10 D; (2) water loss exercise: carrying out a drying process on female adult Neoseiulus barkeri receiving normal feeding with anhydrous calcium chloride for 6 hours (temperature, 25 DEG C; and relative humidity, 5.8%) to make the water loss in the female adult Neoseiulus barkeri being about 20% of the total weight; and (3) glucose feeding: after the water loss exercise, feeding the female adult Neoseiulus barkeri with 20% (w / v) of glucose solution under conditions of 25 DEG C and 100% RH for 8 hours. The invention can effectively improve the tolerance of the female adult Neoseiulus barkeri to co-stress of high temperature and low humidity, and can prolong the survival time of the Neoseiulus barkeri under the conditions of high temperature and low humidity .

Description

technical field [0001] The invention relates to a method for improving the tolerance of Neoseiidi mite under high-temperature and low-humidity co-stress, and belongs to the technical field of biological control of natural enemy reproduction. Background technique [0002] Neoseioides genus Acarina, Parasitica, Phytoseiidae, and Neoseiilis are commercialized predatory mites that are widely used on fruit trees, vegetables, flowers and other plants Control all kinds of mites and pests. [0003] Many studies have found that predatory mites have poor control effect on pests and mites in summer, which can easily lead to outbreaks of pests and mites. In summer, high temperature and low humidity go hand in hand. As the temperature rises in a day, the relative humidity of the leaf surface decreases, and when the plants are under drought stress or the surrounding environment is windy, the leaf surface humidity is close to the atmospheric humidity, while in the hours of the highest te...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/00A23K10/37A23K20/163
CPCA01K67/033A23K10/37A23K20/163A23K50/00Y02P60/87
Inventor 李亚迎刘怀黄吉
Owner SOUTHWEST UNIVERSITY
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