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Bovine-derived composite microecological preparation and application

A technology of compound microecology and preparation, applied in the biological field, can solve rare problems and achieve the effect of promoting growth, increasing content and good effect

Pending Publication Date: 2020-10-02
ANHUI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few reports on cattle-specific probiotics

Method used

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  • Bovine-derived composite microecological preparation and application
  • Bovine-derived composite microecological preparation and application
  • Bovine-derived composite microecological preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Test reagents and consumables

[0040] Test reagents: MRS broth and agar medium, LB broth and agar medium, physiological saline, Gram staining solution, sodium hydroxide, hydrochloric acid, ELISA kit for cattle, commercial Bacillus subtilis.

[0041] Test consumables: blood collection tubes, blood collection needles, test tubes, petri dishes, anaerobic gas bags.

[0042] Experimental animals and groups

[0043] Select 12 healthy weaned Dabie Mountain yellow calves with similar body size, and randomly divide them into 3 groups, namely the control group (CG group), the Bacillus group (BG group), and the experimental group (EG group), with 4 repetitions in each group. Isolation between groups, isolation from the herd, and tie-feeding. Feed twice a day, at 6:00 am and 2:30 pm, respectively, with free access to food, and free water after each feeding. The CG group was fed with normal feed, the daily feed of the BG group was added with Bacillus subtilis powder according to t...

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Abstract

The invention discloses a bovine-derived composite microecological preparation. The microecological preparation comprises a mixed solution of bacillus subtilis and lactococcus lactis, is separated from the body of Dabie Mountain yellow cattle, is specially used for yellow cattle breeds, has no biological safety problem, has a good colonization effect in the intestinal tracts, is screened in a laboratory, and can tolerate the acid environment and the bile salt environment in the animal body. Clinical trials prove that the preparation can promote the growth of calves of the Dabie Mountain yellowcattle, improve serum immune globulin, cytokine and antioxidant indexes, enhance the immune function and antioxidant function of the body, increase the content of beneficial florae in the intestinaltracts and inhibit the reproduction of pathogenic bacteria.

Description

Technical field: [0001] The invention relates to the field of biology, in particular to a bovine-derived compound microecological preparation and its application. Background technique: [0002] With the increase of people's demand for beef, the beef cattle breeding industry has developed rapidly, the scale and density of breeding are increasing, and the environment of livestock houses is poor, which reduces the disease resistance of animals, thereby increasing the risk of disease of animals. Antibiotics The abuse phenomenon of the drug is also becoming more and more serious, and the phenomenon of drug residues in animal products is frequent, which seriously threatens the health of human beings and causes great damage to the environment. Antibiotic-free farming has become an inevitable trend. Therefore, it is very necessary to seek a green additive to replace antibiotics. [0003] Microecological preparations can not only prevent diseases and promote the growth and developme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/10C12R1/125C12R1/01
CPCC12N1/20A23K10/18A23K50/10A23V2400/231
Inventor 王希春谢昕杨克礼杨长根李玉冯士彬汤继顺吴金节
Owner ANHUI AGRICULTURAL UNIVERSITY
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