Method for extracting isorhamnetin 3-o-rutinoside from Suaeda salsa

A technology of Suaeda salsa and rutinoside, which is applied to chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of low purity, high price, difficult extraction and purification, etc., and achieve a safe and simple extraction process, a wide range of The effect of market prospect and wide application value

Active Publication Date: 2021-06-22
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently isorhamnetin 3-O-rutinoside has problems such as low purity, small dose, high price, and difficulty in extraction and purification, which is not conducive to the clinical research of isorhamnetin 3-O-rutinoside

Method used

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  • Method for extracting isorhamnetin 3-o-rutinoside from Suaeda salsa
  • Method for extracting isorhamnetin 3-o-rutinoside from Suaeda salsa

Examples

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Embodiment 1

[0036] Such as figure 1 As shown, the method for extracting isorhamnetin 3-O-rutinoside from Suaeda salsa according to an embodiment of the present invention comprises the following steps:

[0037] S102, using 95% ethanol to reflux for 3 hours to extract Suaeda salsa seed extract;

[0038] S104, concentrating and filtering to obtain mother liquor a, concentrating and drying mother liquor a to obtain sample b;

[0039] S106, using A: water (0.1% TFA) B: acetonitrile as mobile phase, medium pressure preparative liquid phase separation to obtain sample c;

[0040] S108, using A: water (0.1% TFA) B: acetonitrile as mobile phase, preparative liquid phase separation to obtain sample d;

[0041] S110, using methanol as the mobile phase, gel (Sephadex LH-20) separation to obtain isorhamnetin 3-O-rutinoside. The structural formula of isorhamnetin 3-O-rutinoside is as follows figure 2 shown.

[0042] The collected Suaeda salsa seeds were soaked and pretreated before extraction.

Embodiment 2

[0044] S202, seed pretreatment: soak 550 g of Suaeda salsa seeds collected in ultrapure water for 24 hours, and dry at room temperature;

[0045] S204, extracting the seed extract: extracting Suaeda salsa seeds with 95% ethanol under reflux for 3 hours, concentrating to 1L-2L, filtering to obtain mother liquor a, concentrating and drying the obtained mother liquor a, and obtaining about 25 g of sample b;

[0046]S206, sample medium pressure preparation liquid phase separation: Dissolve sample b with a mixture of ethanol and tetrahydrofuran (1:1) and filter, use medium pressure preparation liquid phase separation, the liquid phase mode is Agilent Technologies971-FP FlashPurification System, chromatographic column Daiso ODS-C18 500×80mm, 40-60um, room temperature;

[0047] The mobile phase is ultrapure water (A) and acetonitrile solution (B) containing 0.1% (v / v) formic acid for gradient elution, the elution gradient is 0%-60% 60min, 60%-100% 25min, the flow rate is 70mL·min-1;...

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Abstract

The invention provides a method for extracting isorhamnetin 3-O-rutinoside from Suaeda salsa, comprising the following steps: (1) soaking the collected Suaeda salsa seeds for pretreatment; (2) using 95 Extracting Suaeda salsa seed extract with % ethanol under heat reflux, concentrating and filtering to obtain mother liquor a; (3) concentrating and drying said mother liquor a to obtain sample b; (4) dissolving said sample b with a mixture of ethanol and tetrahydrofuran , after filtration, use medium pressure preparative liquid phase separation to obtain fraction c; (5) dissolve the fraction c with a mixture of water and acetonitrile, and use preparative liquid phase separation after filtration to obtain fraction d; (6) dissolve the fraction c with methanol Fraction d, using methanol as the mobile phase, was separated by gel to obtain solid powder isorhamnetin 3‑O‑rutinoside. Through the technical scheme of the invention, the extraction of isorhamnetin 3-O-rutinoside from Suaeda salsa is realized, the extraction process is safe and convenient, and the purity can reach 99.5%.

Description

technical field [0001] The invention relates to the technical field of natural medicine extraction, in particular to a method for extracting isorhamnetin 3-O-rutinoside from Suaeda salsa. Background technique [0002] Suaeda salsa (Suaeda salsa) belongs to Chenopodiaceae (Chenopodiaceae) annual herbaceous plants, aliases Salsa salsa, Suaeda winged, yellow mustard, widely distributed in North China, Northeast and Northwest China. As a traditional wild vegetable in the north, as early as the famine era, people used Suaeda salsa as food to satisfy their hunger. The stems and leaves of the plant are rich in nutrients, and the growth environment is far away from human habitation, and less polluted by chemical fertilizers and pesticides. It is a typical pollution-free green vegetable and is also a local specialty food. It is used for cold salad, stuffing, stir-fry Food etc. Due to its high nutritional value, the plant is used as a raw material for food and beverages in the food ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 白军红王昕王伟张光亮王大伟
Owner BEIJING NORMAL UNIVERSITY
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