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Imidazolyl-based ionic liquid material, preparation method thereof and application of imidazolyl-based ionic liquid material in phosphorylated peptide enrichment

An ionic liquid and imidazole-based technology, applied in the fields of life sciences and functional materials, can solve the problems of insufficient specific enrichment of phosphorylated peptides, and achieve the effects of increased practicability, good stability, and simple modification methods

Pending Publication Date: 2020-09-22
NINGBO UNIV
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Problems solved by technology

However, the main disadvantage of this kind of early enrichment material is that the specific enrichment of phosphorylated peptides is not strong enough, and acidic peptides are also enriched at the same time, resulting in the signal of the target phosphorylated peptide being greatly inhibited (G.H.Han, M.L. Ye, H.F. Zou, Analyst 2008, 133, 1128-1138.)

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  • Imidazolyl-based ionic liquid material, preparation method thereof and application of imidazolyl-based ionic liquid material in phosphorylated peptide enrichment
  • Imidazolyl-based ionic liquid material, preparation method thereof and application of imidazolyl-based ionic liquid material in phosphorylated peptide enrichment
  • Imidazolyl-based ionic liquid material, preparation method thereof and application of imidazolyl-based ionic liquid material in phosphorylated peptide enrichment

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Embodiment 1

[0022] Embodiment one: wherein A=nSiO 2 .

Embodiment 2

[0023] Embodiment 2: where A=G@mSiO 2 .

[0024] In the above two implementations, the difference lies in the difference of the base material A, and the preparation method is the same: firstly, a ligand containing a phosphoric acid group is prepared, and then the synthesized ligand is used to modify the surface of the base material to obtain an organic phosphonic acid group-modified Nanomaterial A-ImPFIL; after acidification treatment, metal ions are immobilized on organic phosphonic acid groups to obtain immobilized metal ion affinity material A-ImPFIL-Ti 4+ , which is the ionic liquid material in this application.

[0025]The specific preparation method is as follows.

[0026] (1) The synthetic of ligand, its synthetic method is as follows: in 40mL anhydrous toluene, add 2.7g imidazoles, 4.5g 3-chloropropyltriethoxysilane successively, pass into N 2 One minute later, it was stirred and heated to 110° C., and after 3 hours of reaction, 5 g of diethyl (3-bromopropyl) phosph...

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Abstract

The invention discloses an imidazolyl-based ionic liquid material, and application of the imidazolyl-based ionic liquid material in phosphorylated peptide enrichment of a sample containing phosphorylated peptides. A preparation method comprises the following steps: (1) preparing a functionalized imidazolyl ionic liquid containing organic phosphonic acid; and (2) modifying a substrate material withthe functionalized imidazolyl ionic liquid containing organic phosphonic acid. The functionalized material prepared by the invention has better hydrophilicity, metal ion immobilization capability, acid and alkali resistance and stability, has good specific selectivity, and is suitable for enrichment and purification of phosphorylated peptides of complex biological samples. The material has a wideapplication prospect in the field of biomedicine.

Description

technical field [0001] The invention belongs to the field of functional materials and life sciences, and in particular relates to an imidazole-based ionic liquid material, and meanwhile, the invention also relates to a preparation method of the material and its application in enriching phosphorylated peptides. Background technique [0002] Since Williams and Wilkinsons first proposed proteomics in 1994, it has opened a new stage in the development of life sciences and related disciplines. Proteomics studies the composition, physical and chemical properties, and biological properties of proteins; the interaction between proteins; the post-translational modification of proteins, etc. It provides a large amount of information for the mechanism of biological physiological processes, the search and identification of biomarkers, disease diagnosis and treatment, and drug design and development. Protein phosphorylation is a ubiquitous and important post-translational modification i...

Claims

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Application Information

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IPC IPC(8): C07F9/6506G01N1/34G01N1/40G01N1/44
CPCC07F9/6506G01N1/405G01N1/34G01N1/44Y02P20/54
Inventor 王斌斌梁洪泽蒋雨菲梁炜达刘彬蒋银丽卢俐衡林嘉宇桑坤明黎思蕴赵玲玲
Owner NINGBO UNIV
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