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Application of miR-10a-mediated tumor-associated fibroblast to inhibition of hepatic metastasis in colorectal cancer

1. The technology of mir-10a and fibroblasts, which is applied in the direction of antineoplastic drugs, medical preparations containing active ingredients, organic active ingredients, etc., can solve the lack of systematic research and unclear interaction between cancer cells and fibroblasts , affecting colorectal cancer liver metastasis and other issues

Pending Publication Date: 2020-09-18
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, in colorectal cancer, especially in the process of colorectal cancer liver metastasis, the interaction between cancer cells and fibroblasts is still lack of systematic research, whether and how this interaction affects colorectal cancer liver metastasis is still unclear

Method used

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  • Application of miR-10a-mediated tumor-associated fibroblast to inhibition of hepatic metastasis in colorectal cancer
  • Application of miR-10a-mediated tumor-associated fibroblast to inhibition of hepatic metastasis in colorectal cancer
  • Application of miR-10a-mediated tumor-associated fibroblast to inhibition of hepatic metastasis in colorectal cancer

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Embodiment Construction

[0048] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0049] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0050] 1 Experimental method

[0051] 1.1 Cell culture, subculture and cryopreservation

[0052] (1) Reagent preparation:

[0053] ① DMEM medium: 89% DMEM high-glucose medium stock solution + 10% FBS + 1% penicillin and streptomycin double antibodies.

[0054] ②MEM-α medium: 89% MEM-α medium stock solution + 10% FBS + 1% penicillin and streptomycin double antibodies.

[0055] ③ DMEM / F12 primary cell culture medium: 79% DMEM / F12 medium stock solution + 20% FBS + 1% penicillin and streptomycin double antibodies.

[0056] (2) Experimental steps:

[0057] ① Cell line culture: Huma...

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Abstract

The invention provides an application of an miR-10a-mediated tumor-associated fibroblast to a product with at least one of the following functions (1) to (6): (1) inhibiting tumor cell proliferation,(2) promoting tumor cell apoptosis, (3) inhibiting tumor cell migration, (4) inhibiting tumor cell invasion, (5) inhibiting tumor cell sphere formation, and (6) preventing and / or treating a tumor, wherein the tumor is a colorectal cancer; a tumor cell is a colorectal cancer cell; and a nucleotide sequence of miR-10a is as shown in SEQ ID NO:1. According to the application, the effect of the overexpressed miR-10a on liver fibroblast activity in vitro is known and understood from a new perspective, and the effect of a change of the liver fibroblast activity on formation of metastasis in liver bythe colorectal cancer cell is thoroughly discussed, so as to provide a molecular mechanism of hepatic metastasis in the colorectal cancer with an epigenetic basis and to provide a new idea and targetfor prevention and treatment of the colorectal cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of miR-10a mediating tumor-associated fibroblasts to suppress liver metastasis of colon cancer. Background technique [0002] Colon cancer (colon cancer) is one of the most common malignant tumors of the digestive tract in humans. The incidence rate in my country is about 31.39 / 100,000. With the change of people's living habits and food spectrum, the incidence rate shows a clear upward trend. The basic treatment for colon cancer is surgical resection followed by adjuvant chemotherapy for high-risk stage II and III / IV colon cancer patients. Most early colon patients can be successfully surgically resected, but for stage II / III patients, recurrence often occurs, which is closely related to metastasis. About 30% of patients have liver metastases of varying degrees when they are first diagnosed, and about 20% of patients will develop metachronous liver metastases during ...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61K31/7088A61P35/00A61P35/04C12Q1/6886
CPCA61K45/00A61K31/7088A61P35/00A61P35/04C12Q1/6886C12Q2600/178C12Q2600/106
Inventor 章广玲郑璇王梅梅熊亚南
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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