In-vitro cultivated calculus bovis and preparation method thereof

A technology for cultivating bezoar and ox bile in vitro, applied in the field of in vitro cultivating bezoar, can solve problems such as many control links, differences in bile acid content, and insufficient utilization of ox bile resources.

Pending Publication Date: 2020-09-15
WUHAN JIANMIN DAPENG PHARMA
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, due to the different sources of ox bile, the content of bound bile acids in ox bile is quite different, and the ox bile that can meet the technical requirements of cultivating bezoar in vitro is limited, and the resources of ox bile cannot be fully utilized.
On the other hand, it is necessary to control the difference of finished products within a reasonable range, which leads to more control links in the industrial production process, and the control process is more cumbersome.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro cultivated calculus bovis and preparation method thereof
  • In-vitro cultivated calculus bovis and preparation method thereof
  • In-vitro cultivated calculus bovis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] [Example 1] Carry out implementation according to the new process of cultivating Calculus Bovis in vitro of the present invention

[0028]Using 10L of ox bile as raw material, add 1L of 0.02% CTAB solution, stir for 5min, let stand for 30min, filter to remove the precipitate, and obtain 10.95L of filtrate, adjust the pH of the filtrate to 10.3 with sodium hydroxide, add 250ml of 0.02% zinc sulfate solution and 250ml. .01% calcium chloride solution to form a metal salt complex, filter to obtain the filtrate, adjust the pH to 1.5 with concentrated hydrochloric acid, stir for 2 minutes, let stand for 20 minutes, centrifuge to collect 500g of precipitate, take 500g of precipitate and 400g of silica gel (100 mesh) Directly mix the sample and dry to obtain the mixed sample silica gel, with a height of 5 cm, an inner diameter of the silica gel chromatography column of 22 cm, a column packing silica gel (100 mesh) amount of 5 kg, and a height of 35 cm. Elute with ethyl acetate / ...

Embodiment 2

[0033] [Embodiment 2] Carry out implementation according to the new process of cultivating Calculus Bovis in vitro of the present invention

[0034] Using 12L of ox bile as raw material, add 1.2L of 0.02% CTAB solution, stir for 5min, let stand for 30min, filter to remove the precipitate, and obtain 13.15L of filtrate, adjust the pH of the filtrate to 10.3 with sodium hydroxide, add 260ml of 0.02% zinc sulfate solution and 260ml 0.01% calcium chloride solution to form a metal salt complex, filter the filtrate, adjust the pH to 1 with concentrated hydrochloric acid, stir for 2min, let stand for 20min, centrifuge to collect 570g of precipitate, take 570g of precipitate and 400g of silica gel (200 mesh) Directly mix the sample and dry to obtain the mixed sample silica gel, with a height of 5.8 cm, an inner diameter of the silica gel chromatography column of 22 cm, a column packing silica gel (200 mesh) amount of 5 kg, and a height of 35 cm. Elute with ethyl acetate / methanol / formi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an in-vitro cultivated calculus bovis and a preparation method thereof. Fresh fel bovis is used as a raw material, and conjugated bile acid (taurocholic acid, glycocholic acid,taurodeoxycholic acid and glycodeoxycholic acid) with a high purity is obtained by utilizing a modern separation technology. By carrying out technical promotion on an industrialized in-vitro cultivated calculus bovis process and adding the high-purity conjugated bile acid in the cultivation link, the finally prepared in-vitro cultivated calculus bovis finished product not only conforms to the quality standard, but also has total content of the conjugated bile acid of over 4%, is stable in quality, and has basically consistent quality with high-quality natural calculus bovis.

Description

technical field [0001] The invention belongs to the technical field of in vitro cultured bezoar, and in particular relates to a process for producing in vitro cultured bezoar by using modern separation techniques to obtain combined bile acids and in vitro cultured bezoar. Background technique [0002] Bezoar is bovine gallstones. Bile acids are one of its various active ingredients. Bile acids are divided into bound bile acids and free bile acids. Among them, bound bile acids such as taurocholic acid, glycocholic acid, and taurodeoxy Cholic acid and glycodeoxycholic acid have pharmacological effects such as antibacterial and anti-inflammatory, and their content is one of the important indicators to distinguish high-quality or inferior bezoar. [0003] Natural bezoar is a naturally formed product, and there are unavoidable differences in quality, and its combined bile acid content also has a large difference. Contents are shown in Table 1). However, the sources of high-qual...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/413A61K31/575
CPCA61K35/413A61K31/575
Inventor 罗张龙蔡红娇徐冬平石则勇
Owner WUHAN JIANMIN DAPENG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap