Bifidobacterium infantis freeze-dried powder, preparation method and composite protective agent used by bifidobacterium infantis freeze-dried powder

A technology of bifidobacteria infantis and bifidobacteria, applied in the field of food processing, can solve problems such as the decrease of bacterial density

Pending Publication Date: 2020-09-11
科兴生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the lyoprotectant is the most complicated and difficult key factor to choose. At present, the methods for improving the lyophilized survival rate of Bifidobacterium infantis are mostly focused on this aspect. However, the more the protective agent is added, The lower the number of live bacteria per unit mass of bacteria powder, some research results on freeze-dried bacteria powder of Bifidobacteria show that after the high-density bacteria prepared by fermentation of Bifidobacteria undergo freeze-drying, the density of the bacteria basically decreases exponentially, so It is necessary to prepare a freeze-dried powder that can well maintain the number of live bacteria in the bacterial powder

Method used

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  • Bifidobacterium infantis freeze-dried powder, preparation method and composite protective agent used by bifidobacterium infantis freeze-dried powder
  • Bifidobacterium infantis freeze-dried powder, preparation method and composite protective agent used by bifidobacterium infantis freeze-dried powder

Examples

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Effect test

Embodiment 1

[0051] Embodiment 1 of the present invention provides a kind of composite protective agent for the freeze-dried powder of Bifidobacterium infantis, according to the weight percentage of composite protective agent, composite protective agent comprises 20% skimmed milk powder, 1.6% sodium glutamate, 16% Disaccharides and polysaccharides, 2% small molecule polyols, the rest of the phosphate buffer; small molecule polyols are glycerol;

[0052] The disaccharide is trehalose, the polysaccharide is inulin, and the weight ratio of trehalose to inulin is 1:0.82;

[0053] The preparation method of described composite protecting agent comprises:

[0054] (1) Divide the phosphate buffer solution into three parts according to the volume ratio of 1:1:1, dissolve skim milk powder and small molecular polyols in one part of the phosphate buffer solution, and sterilize at 107°C for 15 minutes to obtain liquid A;

[0055] (2) Dissolve the disaccharide and polysaccharide in another phosphate bu...

Embodiment 2

[0059] Embodiment 2 of the present invention provides a kind of composite protective agent for the freeze-dried powder of Bifidobacterium infantis, according to the weight percentage of composite protective agent, composite protective agent comprises 25% skimmed milk powder, 0.5% sodium glutamate, 8% Disaccharides and polysaccharides, 1.5% small molecule polyols, the rest of phosphate buffer; small molecule polyols are glycerol;

[0060] The disaccharide is trehalose, the polysaccharide is inulin, and the weight ratio of trehalose to inulin is 1:0.6;

[0061] The preparation method of the composite protective agent is the same as in Example 1.

Embodiment 3

[0063] Embodiment 3 of the present invention provides a kind of composite protective agent for the freeze-dried powder of Bifidobacterium infantis, according to the weight percentage of composite protective agent, composite protective agent comprises 13% skimmed milk powder, 2% sodium glutamate, 10.5% Disaccharides and polysaccharides, 4% small molecule polyols, the rest of the phosphate buffer; small molecule polyols are glycerol;

[0064] The disaccharide is trehalose, the polysaccharide is inulin, and the weight ratio of trehalose to inulin is 1:0.35;

[0065] The preparation method of the composite protective agent is the same as in Example 1.

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Abstract

The invention relates to the related technical field of food processing, and more particularly provides bifidobacterium infantis freeze-dried powder, a preparation method and a composite protective agent used by the bifidobacterium infantis freeze-dried powder. The invention provides a bifidobacterium freeze-dried powder composite protective agent which comprises the following components in percentage by weight: 10-30% of skim milk powder, 0.1-5.0% of amino acid and salt thereof, 3-25% of disaccharide or / and polysaccharide, 1-5% of micromolecular polyhydric alcohol and the balance of phosphatebuffer solution, wherein a pH value of the composite protective agent is 6.5-8.5. Through the combined action of the specific composite protective agent and an auxiliary agent, the survival rate andthe viable count of the bifidobacterium infantis freeze-dried powder can be remarkably increased, and the problems that the bifidobacterium infantis powder is low in survival rate, small in viable count, short in preservation period and the like in the freeze-drying process are solved.

Description

technical field [0001] The invention relates to the relevant technical field of food processing, and more specifically, the invention provides a freeze-dried powder of Bifidobacterium infantis, a preparation method and a composite protective agent used therefor. Background technique [0002] In recent years, probiotics have been widely used in the fields of functional dairy products, health food, microecological preparations, and medical treatment. Specifically, they refer to a class of active microorganisms that have functional health benefits for the host. They can regulate human immune function, improve intestinal It has the advantages of micro-ecological balance of flora, reduction of blood fat and cholesterol, and promotion of digestion and absorption of nutrients. Common probiotics include lactic acid bacteria, bifidobacteria, Clostridium butyricum and yeast. Bifidobacteria, as the most important physiological bacteria in the intestinal tract of the body, are closely ...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N1/20C12R1/01
CPCC12N1/04C12N1/20
Inventor 马玉涛韩登兰崔宁李国强耿宏伟崔兆臣
Owner 科兴生物制药股份有限公司
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