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A kind of preparation method and application of phosphatidyl glucoside

A technology of phosphatidyl glucoside and phosphatidyl choline, which is applied in the field of preparation of phosphatidyl glucoside, can solve the problems of not studying the effect of neurodegenerative diseases and difficulty in purifying phosphatidyl glucoside, so as to improve brain Aβ aggregation and reduce behavioral awareness. The effects of knowledge function and broad market application prospect

Active Publication Date: 2021-08-13
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the importance of phosphatidylglucosides in the brain is known, the effect of ingested phosphatidylglucosides on neurodegenerative diseases has not been studied
At the same time, due to the limitation of separation technology, it is extremely difficult to purify and obtain high-purity phosphatidyl glucoside

Method used

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  • A kind of preparation method and application of phosphatidyl glucoside
  • A kind of preparation method and application of phosphatidyl glucoside
  • A kind of preparation method and application of phosphatidyl glucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method of preparation of phosphatidyl glucoside, including the following steps:

[0027] (1) 100 g of phosphatidylcholine is sufficiently mixed with the same weight of silica powder;

[0028] (2) 400 g of glucose and phospholipase D (200000U) were dissolved in 10L phosphate buffer;

[0029] (3) After mixing silica gel powder and the phosphate buffer is mixed at a temperature of 40 ° C for 12 h;

[0030] (4) Centrifugation was collected, extracted 3 times with 1L petroleum ether / ethanol solution (4: 1), and stirred for 10 minutes each time it added 800 ml of petroleum ether;

[0031] (5) The petroleum ether phase is combined, and the high-purity phosphatidylmitide is obtained by steaming the organic solvent.

[0032] The above product was detected by HPLC-ELSD method, and the phosphatidyl glucose content was 92.5%.

Embodiment 2

[0034] A method of preparation of phosphatidyl glucoside, including the following steps:

[0035] (1) 100 g of phosphatidylcholine is sufficiently mixed with the same weight of silica powder;

[0036] (2) 600 g of trehalose and phospholipase D (200000U) are dissolved in 10L phosphate buffer;

[0037] (3) After mixing silica gel powder and the phosphate buffer is mixed at a temperature of 40 ° C for 12 h;

[0038] (4) Centrifugation was collected, extracted 3 times with 1L petroleum ether / ethanol solution (4: 1), and stirred for 10 minutes each time it added 800 ml of petroleum ether;

[0039] (5) The organic phase is combined, and the high-purity phosphatidylmitid is obtained by steaming the organic solvent.

[0040] The above product was detected by HPLC-ELSD method, and the phosphatidyl glucose content was 94.6%.

[0041] Among them, the phospholipid amososide content detection process in Example 1 and Example 2 was detected by HPLC-ELSD (Waters1525 Liquid chromatograph, confi...

Embodiment 3

[0042] Example 3: Biological activity evaluation of phosphatidyl glucoside and phosphatidyl oalgosidin

[0043]Phosphatidyl glucoside and phosphatidyl oalgosidin is a phospholipid compound of the Sn-3 binding glucose and trehalose by a phosphate group. The present invention obtains high-purity phosphatidyl glucoside and phosphatidyl glucoside by extracting the preparation, after three months of continuous intake of phosphatidyl glucoside and phosphatidyl glycoside, by detecting APP / PS1 double transgenic Alzheimer Cognitive levels of conditioned mice, Aβ synthesis and Tau protein aggregation, found that it can improve Alzheimerism. The specific experimental steps are as follows:

[0044] Experimental objects: 17-week-old and male APP / PS1 double transgenic mice, SPF level, body weight of 30 ~ 40 grams, 20, routine environmental feeding.

[0045] Experimental grouping: control group (wild-type mice); model group (APP / PS1 mice); phosphatidyl glucoside treatment group (intake of ...

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Abstract

The invention belongs to the technical field of active substance screening, and in particular relates to a preparation method and application of phosphatidyl glucoside. The invention hydrolyzes phosphatidylcholine through phospholipase D, and simultaneously reacts with carbohydrate substrates to generate phosphatidylglucoside. The enzymatic synthesis of phosphatidyl glucosides has been realized, and it has been confirmed that the obtained phosphatidyl glucosides have the ability to prevent and alleviate Alzheimer's disease, and can improve behavioral cognitive impairment.

Description

Technical field: [0001] The present invention belongs to the technical field of active material screening, and more particularly to the preparation method of phosphatidylideamide and its application thereof. Background technique: [0002] Alzheimer'sDisease, AD is a neurological degenerative disease, is currently the most common and destructive dementia, and is identified by the World Health Organization (WHO) as the world's public health. One of the problems affects nearly 50 million people around the world. The hippocampus and cortex of the Alzheimer disorder will shrink, the most important neuropathological markers are excessive phosphorylation of TAU protein and excessive accumulation of neurogenic fibers and amyloid β (Amyloidbeta, Aβ). Pretique deposition. In addition, the main epistemology features of synapses and neurons, reactive nerve glue hyperplasia, oxidation and inflammatory damage. Unfortunately, the current drug treatment of AD is still in the initial stage, provi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/44C07H15/04C07H1/06A61P25/28
CPCA61P25/28C07H1/06C07H15/04C12P19/44
Inventor 薛长湖刘炎峻徐杰姜晓明李兆杰丛培旭王玉明王静凤
Owner OCEAN UNIV OF CHINA
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