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Composite hydrogel, preparation method and application thereof

A composite hydrogel and gel water technology, which is applied in medical science, prosthesis, additive processing, etc., can solve the toxicity of chemical cross-linking agents, the instability of hyaluronic acid, and the inability to promote the complete healing of cartilage defects and the complete regeneration of cartilage and other problems, to achieve the effect of convenient operation, good application prospects, and good cartilage repair

Active Publication Date: 2020-09-04
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These therapies still have major limitations: they cannot promote the complete healing of cartilage defects and the complete regeneration of cartilage
[0011] Due to the instability of hyaluronic acid in the 3D printing bio-ink containing hyaluronic acid in the prior art, it is necessary to add a chemical cross-linking agent. Due to the toxicity of the chemical cross-linking agent, the application of 3D printing ink is limited.

Method used

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  • Composite hydrogel, preparation method and application thereof
  • Composite hydrogel, preparation method and application thereof
  • Composite hydrogel, preparation method and application thereof

Examples

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Embodiment 1

[0049] Weigh 11g of NaOH and 4g of urea into a beaker, add 85g of water to dissolve, and pre-cool to -18°C. Weigh 2g of chitin powder and disperse in it, place it in the freezer of the refrigerator to freeze, and stir it in time during the freezing process to ensure that the chitin is evenly dispersed. After 48 hours, it was taken out and thawed into a clear and translucent aqueous solution. Add 2.9g of 1,2-propylene oxide to 100g of 2% chitin solution at 2°C, raise the temperature to 5°C, react for 24h, then raise the temperature to 15°C, and react for 6h. The pH of the reaction system was adjusted to 7 with HCl to terminate the reaction. Dialyze and freeze-dry to obtain white spongy hydroxypropyl chitin, which is HPCH gel powder.

[0050] Accurately weigh 120.0mg of HPCH gel powder, put it into a 10mL small beaker, add 20mL deionized water into the beaker, stir magnetically for 10min to disperse evenly, and place it in a refrigerator at 8°C overnight. After HPCH is fully ...

Embodiment 2

[0052] The preparation method of the HPCH gel powder in this embodiment is the same as that in Example 1. Accurately weigh 120.0mg of HPCH gel powder, put it into a 10mL small beaker, add 20mL deionized water to the two beakers, stir magnetically for 10min to disperse evenly, and place it in a refrigerator at 8°C overnight. After HPCH is fully swollen, weigh 20 mg HA and add them to two beakers, stir evenly with a glass rod, submerge the bottom of the beakers into an ultrasonic cleaning machine, and ultrasonically mix for 30 minutes. At this time, HA / HPCH gel aqueous dispersions with a mass ratio of 1:6 were prepared. Pour the gel dispersion into a round glass dish, put it in a -20°C refrigerator stably, take it out after 6 hours, put it in a freeze dryer, freeze dry it for 12 hours, and get m(HA) / m(HPCH)=1:6 The gel powder of mass ratio is named as HA / HPCH-1. Accurately weigh 40.0mg of gel powder HA / HPCH-1 in a 5mL centrifuge tube, add 2mL of the above-prepared PBS into the...

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Abstract

The invention discloses composite hydrogel, a preparation method and application thereof, and belongs to the technical field of biology. The preparation method of the composite hydrogel comprises thefollowing steps: dissolving chitin in an alkali and / or urea solution, and carrying out etherification modification on the chitin to obtain hydroxypropyl chitin (HPCH); uniformly mixing the HPCH and hyaluronic acid (HA) to prepare a gel aqueous dispersion, pouring the gel aqueous dispersion in a glass container, placing the glass container in a refrigerator, and performing freeze-drying to obtain gel powder; dissolving the gel powder in a PBS, performing uniform ultrasonic mixing, and then placing the obtained mixture in the refrigerator overnight for swelling; and after swelling, adding powdercontaining Mg<2+>, and performing uniform stirring and then ultrasonic dispersion to obtain Mg<2+> / HA / HPCH composite hydrogel. The composite hydrogel can be used for preparing 3D printing bio-ink, printing in vivo can be realized according to designed shapes and sizes, the sprayed bio-ink can be formed at a specified part, and cartilage defects can be repaired individually.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composite hydrogel, a preparation method and an application thereof. Background technique [0002] Cartilage tissue is an important connective tissue in the human body. Because cartilage tissue lacks vascular distribution, lymphatic drainage, and innervation, and its cells are highly differentiated, its self-repair ability is very limited. Therefore, how to treat cartilage damage is an urgent problem to be solved. [0003] At present, there are many reported methods for the treatment of cartilage defects: arthroscopic debridement, microfracture, chondrocyte / bone marrow mesenchymal stem cell transplantation, autologous / allogeneic cartilage transplantation, joint replacement, etc. These therapies still have major limitations: they cannot promote the complete healing of cartilage defects and the complete regeneration of cartilage. [0004] In recent years, the development of tissue e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/50A61L27/20A61L27/02A61L27/54B33Y10/00B33Y80/00B33Y70/10C08J3/075C08L5/08C08K3/16C08B37/08
CPCA61L27/52A61L27/50A61L27/20A61L27/025A61L27/54B33Y10/00B33Y80/00B33Y70/10C08J3/075C08B37/003A61L2430/06A61L2300/236A61L2300/232A61L2300/412A61L2300/102C08J2305/08C08J2405/08C08K2003/166C08L5/08
Inventor 王洪章其敏金盛阳蒋序林孟春庆何宇黄玮王小红马凯歌王泽
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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