Microbiological detection system

Abstract

The invention relates to a preparation method of a vertical microbiological detection chip. The method comprises the following steps: forming a through and / or non-through microchannel structure on each sheet layer through differentiated mask design; then combining the sheet layer materials to obtain a water conveying channel located on the independent sheet layer, and a containing cavity used forcontaining micro-droplets, or a combined conveying channel and a combined accommodating cavity formed by combining a plurality of sheet layers. The chip prepared by the invention is also provided witha side flow channel capable of inducing the micro-droplets to enter the accommodating cavity; the side flow channel can have the same depth as the accommodating cavity, or is only set in a middle layer, so that the depth is smaller than that of the accommodating cavity; and by setting the microchannel structure in the middle layer to have a hydrophilic property higher than that of the micro-channel structure of other sheet layers, the same lateral flow strength is achieved by using a reduced side flow channel cross-sectional area.

Description

technical field [0001] The invention relates to a biological detection device, in particular to a microbial detection system for performing specific DNA detection of microorganisms such as viruses and bacteria through PCR reactions. Background technique [0002] The digital PCR chip based on micro-droplets is one of the most advanced nucleic acid quantitative detection methods. Compared with traditional PCR technology, digital PCR dilutes the solution containing target genes, primers, polymerases, etc., and divides them into dozens to dozens Tens of thousands of tiny, independent reactors, so that the number of nucleic acid templates in each reactor is less than or equal to 1, and each reactor is subjected to traditional PCR amplification and fluorescence detection. The reactor containing the target gene is marked as 1, and the reactor without the target gene is marked as 0. According to the relative ratio and the volume of the reactor, the nucleic acid concentration of the ...

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Problems solved by technology

However, due to the resistance of the microchannel itself and the local barrier, there is a large pressure difference in the continuous phase fluid flowing between the upstream and downstream of the distribution channel, which will lead to a huge difference in the filling effect of the micro-reaction chamber at the upstream and downstream of the distribution channel.

[0005] In addition, in the process of fluorescence detection of the amplified micro-reaction chamber, the reflection and refraction of the incident excitation light often cause strong background interference; Due to the non-directional nature of the fluorescence emitted by the stimulated micro-droplets in the reaction chamber, some of the fluorescence signals between adjacent micro-wells often pass through the hole wall and are mixed with the fluorescent signals from adjacent droplets, making it difficult to distinguish The problem

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