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Corn microbial seed coating agent and preparation method thereof

A microbial strain and microbial technology, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve the problems of difficult storage of coated seeds, easy film peeling, low coating rate, etc. , to achieve good siderophore production capacity, long activity maintenance time, and the effect of promoting seedling growth

Active Publication Date: 2020-08-25
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing problems of biological seed coating agents mainly include: (1) the function is relatively single and lacks pertinence, and for some special crops or special areas, the effect of commonly used coating agents is average; (2) there are few coating methods , generally all are uniform coatings, and the coating rate is low, and the outer film is easy to fall off, causing the active ingredients inside to flow out, and the active ingredients are greatly affected by the environment, reducing the field effect of the seed coating agent; (3) coated seeds Difficult to store, prolonged storage will reduce the effect of seed coating agent
[0007] Most of the corn seed coating agents currently used in the market are pesticide-type seed coating agents. Many products have problems such as chemical aging, unstable properties, and high coating shedding rate. The application of microorganisms in corn seed coating agents is relatively seldom.

Method used

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  • Corn microbial seed coating agent and preparation method thereof
  • Corn microbial seed coating agent and preparation method thereof
  • Corn microbial seed coating agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 corn microbial seed coating agent of the present invention and preparation method thereof

[0038] 1. The formula of seed coating agent BSCA-1

[0039] Active ingredient: Bacillus Velez TXM-1 bacterial liquid ≥ 10 7 cfu mL -1 ;

[0040] Additives: 100 milliliters of water, add film-forming agent 10g, thickener 0.5g, preservative 0.1g, coloring agent 0.5g; wherein, the film-forming agent is composed of sodium alginate and polyvinyl alcohol, SA:PVA= 1:9, the thickener is bentonite, and the preservative is sodium benzoate;

[0041]The volume ratio of the Bacillus velaisi bacteria liquid to the water in the auxiliary agent is 1:1.

[0042] 2. The formula of seed coating agent BSCA-2

[0043] Active ingredient: Bacillus amyloliquefaciens TXM-2 bacterial liquid ≥10 7 cfu mL -1 ;

[0044] Auxiliaries: 10wt% film-forming agent, 0.5wt% thickener, 0.1wt% preservative, 0.5wt% coloring agent; wherein, the film-forming agent is composed of sodium alginate and po...

Embodiment 2

[0058] Example 2 Determination of characteristics of Bacillus velezensis and Bacillus amyloliquefaciens

[0059] 1. Antagonistic properties

[0060] A total of 11 strains were tested, including 7 strains of Bacillus and 4 strains of Trichoderma, which were TXM-1 (Bacillus amyloliquefaciens), TXM-2 (Bacillus Velez), TXM-3 (Bacillus amyloliquefaciens), TXM-4 (Bacillus pumilus), TXM-5 (Bacillus subtilis), TXM-6 (Bacillus amyloliquefaciens), TXM-7 (Bacillus cereus), TXMT-2 (Trichoderma reesei), TXMT- 3 (T. reesei), TXMT-4 (T. harzianum) and TXMT-5 (T. viride).

[0061] Use a hole puncher to punch holes on the Trichoderma and the pathogenic bacteria, and pick one piece to the center of the two sides of the plate with a distance of 5-6 cm. For Bacillus, use the tip of a pipette to point at a distance of 2 to 3 cm from the pathogen block, and place it in an incubator at 28°C for upside-down cultivation. The pathogen plate cultured alone is used as a control, see Figure 3-4 .

[...

Embodiment 3

[0076] Embodiment 3 contains the performance of the microbial seed coating agent of different film-forming agents

[0077] The microbial seed coating agent in the present embodiment except film-forming agent, all the other compositions are identical with embodiment 1.

[0078] 1. Effects on the growth of corn seedlings

[0079] Soak the corn seeds in 75% ethanol for surface disinfection and air-dry; then coat the corn seeds with microbial seed coating agents containing different compositions of film-forming agents. The film-forming time is all within 5 minutes, dry at room temperature, and finally Place them in germination boxes soaked with two layers of filter paper, and cultivate them at room temperature.

[0080] Each process 10 seeds, three repetitions; After 7 days, calculate germination rate, after 14 days, measure root length, stem length, leaf length, leaf width and seed vigor index (VI), see table 3 and Figure 6 .

[0081] Table 3 Effects of different ratios of fi...

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Abstract

The invention relates to a corn microbial seed coating agent and a preparation method thereof. The corn microbial seed coating agent comprises an active component and an auxiliary agent, Bacillus amyloliquefaciens or Bacillus velezensis is used as the active component, and water serves as a medium of the auxiliary agent. Sodium alginate and polyvinyl alcohol are used as film-forming agents. A thickening agent, a preservative and a coloring agent are added. The obtained corn microbial seed coating agent does not contain a bactericide, has a good growth-promoting effect while antagonizing the growth of pathogenic bacteria, can promote the germination of corn seeds and promote the growth of corn in the seedling stage, is efficient, low in toxicity, safe and reliable, meets the development requirements of green agriculture, is simple and feasible in preparation method, and has important practical value.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a corn microbial seed coating agent and a preparation method thereof. Background technique [0002] Corn is one of the main grains in my country. It has high economic development prospects and research value because it is rich in crude fiber, vitamins, trace elements and a large amount of protein. With the improvement of the important position of corn, improving the quality and total yield of corn has become an important research direction of my country's agricultural development. [0003] One of the important reasons affecting maize yield is the infection of soil-borne diseases, and inhibiting the occurrence of diseases has become the key to improving maize yield. Seed coating agent is a combination of one or more active ingredients, binders and colorants. play an important role. [0004] At present, the application range of seed coating technology is rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01P3/00A01P1/00
CPCA01N63/22
Inventor 黄艳娜唐雪明王金斌周茂超宋丽莉吴潇吕贝贝
Owner SHANGHAI ACAD OF AGRI SCI
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