Micro-fluidic detection chip and detection method thereof

Abstract

The invention provides a micro-fluidic detection chip, and belongs to the technical field of in vitro diagnosis. The micro-fluidic detection chip comprises a micro-fluidic chip and reagent plugs. Themicro-fluidic chip comprises a sample loading area, a waste liquid area and a stirring and uniformly-mixing channel. The sample loading area comprises a quantitative sample inlet hole, a sample loading sufficient identification hole and a sample loading channel, and the quantitative sample inlet hole is communicated with the sample loading sufficient identification hole. The sample loading channelis communicated with the stirring and uniformly-mixing channel, and a plurality of reagent holes are formed in the stirring and uniformly-mixing channel. Each reagent plug comprises a positioning surface, a reagent accommodating cavity and a plug body. The reagent accommodating cavities are positioned at the upper ends of the plug bodies, and the positioning surfaces are positioned at the lower ends of the plug bodies. The reagent plugs correspond to the reagent holes in position and are inserted into the reagent holes. No liquid reagent component exists in the micro-fluidic chip, so that theprocessing, manufacturing and transportation costs are greatly reduced.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a microfluidic detection chip and a detection method thereof. Background technique [0002] In the field of in vitro diagnostics, in vitro diagnostic products are mainly composed of diagnostic equipment (instruments) and diagnostic reagents, and for all in vitro diagnostic products, especially those with diagnostic reagents, the design and production of reagent status have always been the responsibility of field products. top priority. For almost all IVD products, the main purpose of the reagents is to provide a uniform liquid-phase reaction environment for the entire biological or chemical reaction system, and at the same time combine the knowledge of biology, chemistry and laboratory medicine to "treat" the target substance in the sample. (For example: staining specific cells, forming immune complexes with specific proteins through immune complexation reactions, etc...

AI Technical Summary

Problems solved by technology

Increased processing, manufacturing and transportation costs (requires refrigeration) due to the small amount of encapsulation required (typically tens of microliters)

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