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Shewanella algae scs-1 and application thereof in microbial electrogenesis

A technology of Shewanella, scs-1cgmccno.18696, applied to the seaweed Shewanella scs-1 and its application in microbial electricity production, can solve the problem that strains affect electricity generation ability, limit the application of microbial electricity generation, etc. question

Active Publication Date: 2020-08-18
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the currently discovered electrogenic microorganisms, the ability of microorganisms to generate electricity limits the industrial application of microbial electricity production, and the performance of strains fundamentally affects their electricity production capabilities

Method used

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  • Shewanella algae scs-1 and application thereof in microbial electrogenesis
  • Shewanella algae scs-1 and application thereof in microbial electrogenesis
  • Shewanella algae scs-1 and application thereof in microbial electrogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Obtaining of the algae Shewanella algae scs-1.

[0020] The sea mud and seawater of the present invention are all collected in the ocean near China's Hainan Island, and the samples are stored in a 4 ° refrigerator. The collected samples are enriched by assembling a microbial fuel cell. The battery is a bipolar chamber H type, wherein the anode chamber (working volume 140mL) and cathode chamber (working volume 140mL), separated by DuPont Nafion 117 proton exchange membrane; anode using pretreated carbon cloth (2.5cm×2.5cm), cathode using pretreated carbon cloth (2.5cm×3cm) . Place 60ml of anolyte (see Table 1), 60ml of seawater, and 20ml of sea mud in the anode chamber, add 140ml of catholyte (see Table 2) to the cathode chamber of the battery, connect a 2000 ohm resistor to form a closed loop, and place it in 30°C for incubation Cultivate statically in the box, and use a digital multimeter to measure the output voltage of the microbial fuel cell (such as image 3 show...

Embodiment 2

[0028] Screening of optimal substrates for Shewanella algae scs-1

[0029] Configure basal medium containing different substrates, add the medium to a 96-well plate, inoculate 5% of the seaweed Shewanella algaescs-1 seed solution, and place it at a speed of 200 rpm and a temperature of 30°C cultured in a shaker, and measured its OD600 at 24 hours using a microplate reader. Wherein the size of OD600 has reflected the growth condition of bacterial strain under this substrate, (as figure 2 shown), figure 2 It is the substrate utilization of Shewanella algae scs-1 and the growth curve under the optimal substrate. Among them, A is the OD600 of 25h when 20mM amino acid is used as substrate; B is the OD600 of 25h when 20mM organic acid is used as substrate; C is 25h of growth when 20mM carbohydrate is used as substrate OD600 of D; D is the growth curve under the condition that the optimum substrate lactic acid concentration is 20mM. After experiments, the optimal substrate of S...

Embodiment 3

[0034] Application of Shewanella algae scs-1 in Seaweed Production of Electricity

[0035] Take out the screened strains from the -80°C refrigerator, activate by streaking, inoculate the activated bacteria into LB liquid medium containing 3.4% sodium chloride, and place them in a shaker with a rotation speed of 200 rpm and a temperature of 30°C Cultivate overnight in medium to obtain primary seed solution.

[0036] The primary seed liquid was transferred to 400 ml of anolyte shown in Table 1 for cultivation. The anolyte used sodium lactate as the electron donor, and cultivated it in a shaker at a speed of 200 rpm and a temperature of 30°C for 16 hours to obtain a fermented liquid.

[0037] Start the dual-chamber microbial fuel cell, the anode area in the cell is 1*1cm 2 , the cathode area is 2.5*3cm 2 , adjust the OD600 of the obtained fermented liquid to 1 with fresh anolyte to obtain a diluted liquid. Take 140ml of diluted solution and add it to the anode chamber, take 1...

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Abstract

The invention discloses a shewanella algae scs-1 and an application thereof in microbial electrogenesis. The shewanella algae scs-1 is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 16, 2019, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation number isCGMCC No.18696. The strain has strong electricity generation capacity, when 20 mM of lactic acid serves as an electron donor, the maximum output voltage of the strain is 375+ / -10 mV, and the maximum power density of the strain is 470+ / -15 mW / m<2>.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a strain of algae Shewanella algae scs-1 and its application in microbial electricity production. Background technique [0002] Microbial electricity generation is a new way of energy production. Basic research on electricity-producing microorganisms has become a hot spot in the fields of environmental chemical engineering and energy. Microbial electricity generation is used in wastewater treatment and energy recovery, environmental restoration, hydrogen production by microbial electrolytes, and microbial electrosynthesis. , precious metal reduction, seawater desalination, sludge disposal, nanomaterial synthesis, environmental online detection and many other aspects have shown good application prospects. For the currently discovered electrogenic microorganisms, the ability of microorganisms to generate electricity limits the industrial application of microbial elect...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M4/90H01M8/16C12R1/01
CPCH01M4/90H01M4/9008H01M8/16C12R2001/01C12N1/205Y02E60/50
Inventor 宋浩陈媛媛李锋王磊
Owner TIANJIN UNIV
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