New application of peroxidase acyl coenzyme A oxidase 1

Abstract

The invention belongs to the technical field of antiviral drugs and discloses new application of peroxidase acyl coenzyme A oxidase 1. Firstly, an insect baculovirus expression vector system is used for expressing ACOX1 protein in an insect cell SF9, then a nickel column affinity chromatography method is used for purifying the ACOX1 protein, and finally the antiviral effect of the ACOX1 protein isverified in malignant embryo rhabdomyoma cells RD infected with EV71. Experimental results prove that the purified ACOX1 protein can inhibit EV71 virus replication at the mRNA level and the protein level at a certain concentration. According to the invention, the anti-EV71 virus effect of peroxidase acyl-CoA oxidase 1 (ACOX1) is evaluated from cell and molecular levels, and a good foundation is laid for further development and application. It is shown that the medicine has the prospect of being developed into an effective medicine for resisting EV71 viruses to be applied in clinic.

Description

technical field [0001] The invention belongs to the technical field of antiviral drugs, and in particular relates to a new application of peroxidase acyl-CoA oxidase 1. Background technique [0002] Currently, Human enterovirus 71 (Human enterovirus, EV71) belongs to the genus Enterovirus of the Picomaradae family. The EV71 virus particle is an icosahedral symmetrical spherical structure without envelope and spikes, and its diameter is about 24–30nm. The full length of the viral genome is 7408bp, which is a single-stranded positive-sense RNA. There is only one open reading frame (ORF) in the genome, and there are 5' and 3' non-coding regions (UTRs) on both sides of the ORF, and at the end of the 3' end of the genome There is also a polyadenylic acid tail (poly-A) of variable length. The ORF in the viral genome can encode 2194 amino acids, and the polyprotein composed of these amino acids can produce a total of three precursor proteins, P1, P2 and P3. Among them, the P1 pr...

AI Technical Summary

Problems solved by technology

[0007] Through the above analysis, the problems and defects of the prior art are: (1) there is no relevant report about the antiviral effect of peroxisome and ACOX1 on EV71

[0008] (2) Currently there is no effective antiviral drug to treat severe EV71 infection;

[0009] (3) The vaccine can only prevent but not treat EV71 infection, and the preventive effect cannot reach 100%.

[0010] The difficulty of solving the above problems and defects is: the development of antiviral drugs is a time-consuming and costly work. Although many pharmaceutical companies and research institutions have carried out research and development work for many years, so far there is no new anti-EV71 drug on the market.

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