SSR Molecular Marker Primer Set and Its Application for Sex Identification of Populus niger

A molecular marker and primer set technology, applied in the field of molecular genetics and breeding, can solve the problems of inability to effectively manage the sex of poplar seedlings, fly floc pollution, etc.

Active Publication Date: 2022-05-31
NANJING FORESTRY UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in the process of afforestation and popularization of Black Populus americana, due to the lack of technical methods for gender identification at the seedling stage, it is impossible to effectively manage the sex of black poplar seedlings. Once female plants are mixed in, it will bring Fly floc pollution

Method used

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  • SSR Molecular Marker Primer Set and Its Application for Sex Identification of Populus niger
  • SSR Molecular Marker Primer Set and Its Application for Sex Identification of Populus niger
  • SSR Molecular Marker Primer Set and Its Application for Sex Identification of Populus niger

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Embodiment 1

[0036] (1) Fine mapping of sex-determining loci in Populus americana

[0037] In 2012, the American black poplar hybrid F 1 The population, including 1077 hybrid offspring, was planted in Sihong Forest Farm. As of 2019, all hybrid offspring have reached sexual maturity. Obtain the sex phenotype of the hybrid offspring. First, 72 progeny individuals were randomly selected in the hybrid population for preliminary location of sex-determining loci. As a result, sex-determining loci were located in the proximal telomeric region of chromosome 19, and only the sex locus and SSR locus N110 were detected A recombinant single plant ( figure 1 A). Then, SSR primers were developed in the target interval, and all 1077 individuals of the hybrid progeny were used to fine-map the sex loci. Results The sex-determining locus of P. americana was located in the 236Kb interval of chromosome 19 near the telosome, and four recombinant individuals were detected between the sex-determining locus ...

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Abstract

The invention discloses an SSR molecular marker primer set for identifying the sex of Populus nigra and its application, and belongs to the technical field of molecular genetic breeding. This application uses the extracted Populus americana genomic DNA as a template, and uses the primer pairs of SSR molecular markers NJFU582, NJFU600 and NJFU602 to carry out PCR amplification. band, the black poplar to be tested is a male plant, otherwise it is a female plant. During the breeding and cultivation management of Populus americana, DNA can be extracted from leaves and other vegetative tissues for gender identification at the seedling stage, which can be used for early selection of non-flying male plants of P. Monitor and manage the sex of seedlings, so as to effectively control the problems caused by flying catkins after the seedlings reach sexual maturity.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic breeding, in particular to a SSR molecular marker primer set for identifying the sex of Populus nigra and its application. Background technique [0002] Poplar (Populus spp.) is one of the fast-growing timber tree species with the widest distribution and largest cultivation area in the world, and it plays an important role in economic development and environmental construction. my country's poplar plantation area is about 90 million mu, ranking first in the world. In addition, there are 37.5 million mu of poplar natural forest in my country. American black poplar (Populus deltoides Marsh.) is a species of Populus (Populus) black poplar tree species, native to North America, distributed in the western, central and southwestern United States, the southwestern tip of Canada and northwestern Mexico. Black poplar is a fast-growing tall tree and one of the important fast-growing timber specie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6879C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/6879C12Q1/686C12Q2600/156Y02P60/40
Inventor 尹佟明吴怀通邱宇
Owner NANJING FORESTRY UNIV
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