Wireless transmission multichannel PCR analyzer calibration device and calibration method thereof

Abstract

The invention relates to a wireless transmission multichannel PCR analyzer calibration device and a calibration method thereof. A wireless transmission technology is adopted, workers do not need to bein a high-risk environment for a long time. A plurality of temperature sensors are integrated together by adopting a packaging structure, and the multichannel temperature sensor integrated device ismanufactured. The packaging structure enables the temperature sensor to be immersed in a liquid soaking environment, the liquid soaking environment is used for calibrating the temperature sensor of the calibration device, value tracing and self-calibration during calibration are achieved, errors of the temperature sensor are eliminated and reduced, and it is guaranteed that the calibration resultis accurate and reliable. Therefore, the problems of quantity traceability and self-calibration of the temperature sensor for calibration of the polymerase chain reaction analyzer are solved.

Description

technical field [0001] The invention relates to the technical field of calibration and verification of polymerase chain reaction analyzers, in particular to a wireless transmission multi-channel PCR analyzer calibration device and a calibration method thereof. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as special DNA replication outside the body. The biggest feature of PCR is that it can greatly increase the amount of DNA in a small amount. . Therefore, whether it is ancient organisms in fossils, the remains of historical figures, or the hair, skin or blood left by the murderer in a murder case decades ago, as long as a little bit of DNA can be isolated, it can be amplified by PCR for comparison. right. [0003] Semi-conservative DNA replication is an important way of biological evolution and generation. Double-stranded DNA can be denatured and unwound into a sin...

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Problems solved by technology

[0005] At present, when calibrating the polymerase chain reaction analyzer, it is often necessary to manually insert the temperature sensor into the reaction test tube, manually collect and record the temperature of the reaction test tube, and then according to JJF1527-2015 "Calibration Specification for Polymerase Chain Reaction Analyzer" Calibration of temperature indicator error, temperature uniformity and other items, however, the application environment of polymerase chain reaction analyzer is mostly high-risk environment or ultra-clean laboratory, manual close-range operation, there is a certain risk

[0006] In addition, in order to ensure the accuracy of the calibration results of the polymerase chain reaction analyzer, the first choice is to ensure the accuracy of the calibration equipment used, then the temperature sensor needs to be calibrated regularly to trace the source of the temperature sensor and self-calibrate. In order to ensure that the measurement error of the calibration equipment meets the requirements of use, however, the soaking environment used by the existing polymerase chain reaction analyzers is a solid soaking environment, while the existing temperature value traceability system uses a liquid soaking environment. Therefore, the existing polymerase chain reaction analyzer calibration method lacks the corresponding traceability and self-calibration steps, and the accuracy of the calibration results can only be relied on by the staff. The collected temperature data is judged by experience, and the accuracy of the calibration results is poor

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