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Duck hepatitis A virus universal detection kit

A technology of duck hepatitis A virus and detection kit, which is applied in the determination/inspection of microorganisms, resistance to vector-borne diseases, DNA/RNA fragments, etc., to achieve good specificity

Inactive Publication Date: 2020-08-07
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant research report on the duck hepatitis A virus universal detection kit at home and abroad, and the establishment of the present invention can fill in the gaps in related fields at home and abroad

Method used

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  • Duck hepatitis A virus universal detection kit
  • Duck hepatitis A virus universal detection kit

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Embodiment 1

[0028] 1. Related test pathogens

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Abstract

The invention provides a duck hepatitis A virus universal detection kit and a detection method thereof. The kit comprises a universal primer pair for three types of duck hepatitis A viruses (DHAV-1, DHAV-2 and DHAV-3), and the sequences of the primer pair are shown as SEQ ID NO.1-2. The specific primers adopted by the invention can be used for effectively amplifying the three types of duck hepatitis A viruses (DHAV-1, DHAV-2 and DHAV-3), and can be used for quickly judging whether the duck hepatitis A viruses (DHAV-1, DHAV-2 and DHAV-3) exist in a sample to be detected or not. At present, no related research report of the duck hepatitis A virus universal detection kit exists at home and abroad, and the establishment of the kit provided by the invention can fill the blank in related fieldsat home and abroad.

Description

technical field [0001] The invention provides a universal detection kit for duck hepatitis A virus, which belongs to the field of veterinary epidemiology. Background technique [0002] The International Committee on Taxonomy of Viruses (ICTV) in 2014 (EC 46, Kingston and Montreal, Canada, July 2014, Emailratification 2015 (MSL #29)) synthesized international research on duck hepatitis A virus (duck hepatitis A virus1- 3. The latest research progress of duck hepatitis A virus type 1 DHAV-1, duck hepatitis A virus type 2 DHAV-2 and duck hepatitis A virus type 3 DHAV-3), the duck hepatitis A virus was renamed as avian hepatitis A virus (Avihepatovirus A), in Viruses belong to the genus Avihepatovirus of the family Picornaviridae in taxonomy. In order to facilitate the unification of documents and the research habits of relevant researchers, it is still called duck hepatitis A virus (DHAV) in the present invention. [0003] Genomics analysis found that the complete genome of d...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/706C12Q2565/125Y02A50/30
Inventor 万春和黄瑜陈翠腾傅光华程龙飞施少华陈红梅傅秋玲刘荣昌
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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