Breast cancer organoid culture kit

Abstract

The invention relates to the fields of cytobiology and cell culture, and particularly relates to a breast cancer organoid culture kit. A method for realizing the kit comprises the following steps: (1)preparing, sub-packaging and using a breast cancer organoid culture medium A; (2) preparing, sub-packaging and using a breast cancer organoid culture medium B; and (3) preparing, sub-packaging and using a sample preserving fluid. The breast cancer organoid culture medium A is a DMEM / F12 culture medium containing 100 U / ml of penicillin, 0.1 mg / ml of streptomycin, 1% of HEPES and 1% of Gluta Max 100 X; the organoid culture medium B is a DMEM / F12 culture medium containing EGF, an FGF growth factor, an ALK inhibitor A83-01, Noggin, an HEPES buffer solution, Y27632 and one or more of a Wnt signalpath related secretory protein family R-Spondin 1-4; and the sample preserving fluid is a DMEM / F12 culture medium containing 100 U / ml of penicillin, 0.1 mg / ml of streptomycin and 1% of L-glutamine. The three components form the breast cancer organoid culture kit.

Description

Technical field: [0001] The invention belongs to the field of cell culture, in particular to a human breast cancer tissue organoid culture kit. Background technique: [0002] Cancer is a major public health problem that seriously threatens people's health. The incidence and mortality of cancer in my country are increasing year by year, causing a major economic burden to families and society, and it is also a major "pain point" for people's livelihood in today's society. Although a large number of anti-tumor drugs are launched every year, the choice of clinical treatment plan mainly depends on various evidence-based guidelines and personal experience of doctors, and cancer is a highly heterogeneous disease. Different patients, different parts, and even internal tumors There is a high degree of diversity signatures that determine cancer progression and response to therapy in different patients. How to better understand the characteristics of tumor heterogeneity, match the bes...

AI Technical Summary

Problems solved by technology

[0005] The general organoid culture method is based on the construction of cell line cells, or in vitro culture using tumor tissue from animal models. The whole process is relatively complicated, and more than a dozen cytokines and regulatory factors need to be added to the culture medium used. Both need to be dissolved and prepared during use. On the one hand, pollution and preparation errors are prone to occur. On the other hand, with the development of cell biology technology and the discovery of new cell growth regulation signaling pathways, it is necessary to carry out the original medium formula. Continuous improvement, and the success rate of general medium culture is low (less than 70%), the culture time is long (6-8 weeks), and the operation steps are cumbersome

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