Bifidobacterium animalis subsp. Lactis i797, purification method and application thereof
A technology for separation and purification of animal bifidobacteria, applied in the field of bioengineering, can solve the problems of intestinal loss of the ability to reproduce probiotics and dependence
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Embodiment 1
[0048] Example 1 Bifidobacterium lactis subsp. i797
[0049] This example provides an animal Bifidobacterium milk subsp. i797, which is isolated and screened from the feces of breastfed infants or young children. General Microbiology Center, the preservation address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18403, and the Latin name is Bifidobacterium animalis subsp. lactis .
[0050] The 16SrRNA sequence of the above-mentioned Bifidobacterium animalis subsp. lactis i797 strain is as follows:
[0051] ACGGCTCCCCCACAAGGGTCGGGCCACCGGCTTCGGGTGCTACCCACTTTCATGACTTGACGGGCGGTGTGTACAAGGCCCGGGAACGCATTCACCGCGGCGTTGCTGATCCGCGATTACTAGCGACTCCGCCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGACCGGTTTTCAGCGATCCGCCCCACGTCACCGTGTCGCACCGCGTTGTACCGGCCATTGTAGCATGCGTGAAGCCCTGGACGTAAGGGGCATGATGATCTGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCGGTCCCACATGAGTTCCCGGCATCACCCGCTGGCAACATGCGGCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACGACCATG...
Embodiment 2
[0054] Example 2 A method for separation and purification of Bifidobacterium lactis subsp. i797
[0055] This embodiment provides the separation and purification method of embodiment 1, which is carried out according to the following sequence of steps:
[0056] 1. Collect samples
[0057] Take 1g of intestinal feces from infants or young children, then add it to 9ml of normal saline and mix well to obtain sample A;
[0058] 2. Sample enrichment
[0059] Take V 1 = 2ml sample A, added to V 2 =100mL modified MRS liquid medium, at T 1 = 37 ℃ anaerobic culture t 1 =72h, obtain culture medium B;
[0060] 3. Isolation and screening of strains
[0061] Take 1ml of culture medium B1 and dilute it with 0.9% sterile saline in a 10-fold gradient, followed by a 10-fold gradient dilution. -1 、10 -2 、10 -3 、10 -4 、10 -5 times, corresponding to the bacterial suspension C1 1 ~C1 5 ;
[0062] Take the improved MRS solid medium, after melting, pour it into the first to fifth cult...
Embodiment 3-6
[0071] Embodiment 3-6 A kind of separation and purification method of animal Bifidobacterium lactis subsp. i797
[0072] Embodiment 3-6 is respectively the separation and purification method of embodiment 1, is basically the same as the method of embodiment 2, and difference is that the technical parameter of separation and purification process is different, and specific parameters are as shown in table 1:
[0073] Table 1 Example 3-6 Separation and purification process and parameters
[0074]
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