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Bifidobacterium animalis subsp. Lactis i797, purification method and application thereof

A technology for separation and purification of animal bifidobacteria, applied in the field of bioengineering, can solve the problems of intestinal loss of the ability to reproduce probiotics and dependence

Active Publication Date: 2020-08-04
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, medical research has shown that if artificially synthesized probiotics are used for a long time, the intestinal tract may gradually lose its ability to reproduce probiotics, making the human intestinal tract dependent on artificial probiotics, resulting in "probiotic dependence"

Method used

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  • Bifidobacterium animalis subsp. Lactis i797, purification method and application thereof
  • Bifidobacterium animalis subsp. Lactis i797, purification method and application thereof
  • Bifidobacterium animalis subsp. Lactis i797, purification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Bifidobacterium lactis subsp. i797

[0049] This example provides an animal Bifidobacterium milk subsp. i797, which is isolated and screened from the feces of breastfed infants or young children. General Microbiology Center, the preservation address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18403, and the Latin name is Bifidobacterium animalis subsp. lactis .

[0050] The 16SrRNA sequence of the above-mentioned Bifidobacterium animalis subsp. lactis i797 strain is as follows:

[0051] ACGGCTCCCCCACAAGGGTCGGGCCACCGGCTTCGGGTGCTACCCACTTTCATGACTTGACGGGCGGTGTGTACAAGGCCCGGGAACGCATTCACCGCGGCGTTGCTGATCCGCGATTACTAGCGACTCCGCCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGACCGGTTTTCAGCGATCCGCCCCACGTCACCGTGTCGCACCGCGTTGTACCGGCCATTGTAGCATGCGTGAAGCCCTGGACGTAAGGGGCATGATGATCTGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCGGTCCCACATGAGTTCCCGGCATCACCCGCTGGCAACATGCGGCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACGACCATG...

Embodiment 2

[0054] Example 2 A method for separation and purification of Bifidobacterium lactis subsp. i797

[0055] This embodiment provides the separation and purification method of embodiment 1, which is carried out according to the following sequence of steps:

[0056] 1. Collect samples

[0057] Take 1g of intestinal feces from infants or young children, then add it to 9ml of normal saline and mix well to obtain sample A;

[0058] 2. Sample enrichment

[0059] Take V 1 = 2ml sample A, added to V 2 =100mL modified MRS liquid medium, at T 1 = 37 ℃ anaerobic culture t 1 =72h, obtain culture medium B;

[0060] 3. Isolation and screening of strains

[0061] Take 1ml of culture medium B1 and dilute it with 0.9% sterile saline in a 10-fold gradient, followed by a 10-fold gradient dilution. -1 、10 -2 、10 -3 、10 -4 、10 -5 times, corresponding to the bacterial suspension C1 1 ~C1 5 ;

[0062] Take the improved MRS solid medium, after melting, pour it into the first to fifth cult...

Embodiment 3-6

[0071] Embodiment 3-6 A kind of separation and purification method of animal Bifidobacterium lactis subsp. i797

[0072] Embodiment 3-6 is respectively the separation and purification method of embodiment 1, is basically the same as the method of embodiment 2, and difference is that the technical parameter of separation and purification process is different, and specific parameters are as shown in table 1:

[0073] Table 1 Example 3-6 Separation and purification process and parameters

[0074]

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Abstract

The invention discloses bifidobacterium animalis subsp. Lactis i797 which is preserved in China General Microbiological Culture Collection Center on August 20, 2019, the preservation address is No.3,Yard 1, Beichen west road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.18403. The invention also discloses a preparation method of the bifidobacterium animalis subsp. Lactis i797. The invention further discloses a separation and purification method and application of the bifidobacterium animalis subsp. Lactis i797. The bifidobacterium animalis subsp. Lactis i797 provided bythe invention can adjust intestinal flora balance and improve fecal characteristics, and has a better simulated digestive juice survival rate; and the post-acid can be well controlled under the preservation condition of a high temperature suitable for the growth of lactic acid bacteria at 37 DEG C. The invention belongs to the technical field of bioengineering, and provides a bifidobacterium animalis subsp. Lactis i797 which has the effect of regulating intestinal flora balance and can be used in the process of making foods, medicines or beverages.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a bacterial strain, a screening method and an application, in particular, bifidobacterium subsp. lactis i797, its separation and purification method and application. Background technique [0002] Bifidobacterium was first discovered by Henry Tissier in 1899 when it was isolated from the feces of breastfed infants. Bifidobacterium is a Gram-positive bacillus, which is very sensitive to oxygen, has poor tolerance to low pH, and is easily inactivated in a low pH environment; its optimum pH is 6.5-7.0, and its optimum growth temperature is 37-42°C. Bifidobacteria widely exist in the digestive tract, vagina and oral cavity of humans and animals, and are one of the important members of the intestinal flora of humans and animals. It has been found that there are 32 subtypes of Bifidobacteria, and there are as many as 70 biological agents containing Bifidobacteria. [0003] Bifido...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23L2/38A23C9/123A23L7/104A23L13/40A23L13/70A23L33/135A61K35/745A61P1/00C12R1/01
CPCC12N1/20C12N1/02A23L2/382A23C9/1234A23L7/104A23L13/45A23L13/74A23L33/135A61K35/745A61P1/00A23V2002/00C12R2001/01C12N1/205A23V2400/515A23V2200/3204Y02A40/90A23L13/30A23V2200/3202
Inventor 冯丽莉张栋朱宏王世杰荀一萍薛玉玲康志远韩晓伟
Owner JUNLEBAO DAIRY GRP CO LTD
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