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Preparation device and method of skin cell suspension liquid

A skin cell and skin technology, applied in the preparation of skin cell suspension and its preparation field, can solve the problems of complicated operation process and inconvenient use, and achieve simple operation process, large coverage area, and reduce microbial contamination such as bacteria risk effect

Pending Publication Date: 2020-07-31
李洪生
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing skin cell suspension preparation is carried out in the laboratory, and the preparation process is as follows: put the skin tissue to be digested into a centrifuge tube, add digestive enzyme solution, place it in a 37°C water bath or incubator, and shake continuously After the digestion is completed, filter, centrifuge the liquid, discard the supernatant, add Hanks solution or serum-free culture medium, centrifuge and rinse for 1-2 times, remove the supernatant, add culture medium to make a cell suspension, the operation process is more complicated and requires special It can only be completed with qualified personnel and equipment, and it is extremely inconvenient to use clinically

Method used

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  • Preparation device and method of skin cell suspension liquid
  • Preparation device and method of skin cell suspension liquid
  • Preparation device and method of skin cell suspension liquid

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Embodiment 1

[0039] like Figure 1-3 Shown, the present embodiment provides a kind of preparation device of skin cell suspension, comprises following parts:

[0040] The preparer housing and the operating table arranged on the preparing device housing, the operating table is provided with a digestion tank 7, a washing tank 8, a suspension preparation tank 9 and a display 12, and the digestion tank 7 is used for digesting Skin tissue, the washing pool 8 is used to wash digested skin tissue, and the suspension preparation pool 9 is used to prepare skin cell suspension;

[0041] A circuit control component and a heating component are arranged in the housing of the preparation device, the circuit control component is used to control the heating temperature and time of the digestion tank 7, and the heating component is used to heat the digestion tank 7;

[0042] The first filter and the second filter are respectively detachably arranged in the digestion tank 7 and the suspension preparation ta...

Embodiment 2

[0051] The present embodiment provides a kind of preparation method of skin cell suspension, comprises the steps:

[0052] (1) Inject 10 ml of sterile water into a bottle containing 2.5 mg of trypsin, dissolve and mix and inject into the first filter of the digester 7, press the heating button 11, and wait until the temperature reaches 37 After the ℃ display light turns green, add a small piece of skin tissue (skin tissue, thickness 0.1-0.2mm, washed with normal saline and cut into 2-5mm size), press the timing button 13 to time, until the skin tissue becomes fluffy and flocculent After the shape (about 25 minutes), the digested skin tissue was obtained;

[0053] (2) Take out the first filter in the digestion tank 7, discharge the liquid in the first filter, then repeatedly extract the enzyme in the washing tank 8 to stop the washing liquid, stop the enzyme digestion reaction, and separating the tissue cells, sucking the first filter, draining the liquid, and obtaining the se...

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Abstract

The invention relates to a preparation device and method of skin cell suspension liquid. The preparation device comprises a preparation device shell, an operation table arranged on the preparation device shell, a first filter and a second filter, wherein a digestion tank, a washing tank and a suspension liquid preparation tank are arranged on the operation table; the digestion tank is used for digesting skin tissues; the washing tank is used for washing the digested skin tissues; the suspension liquid preparation tank is used for preparing the skin cell suspension liquid; the first filter andthe second filter are detachably arranged in the digestion tank and the suspension liquid preparation tank respectively; each of the first filter and the second filter comprises an outer sleeve and aninner core barrel; and the outer sleeves annularly sleeve the inner core barrels. By means of the device, a clinician can rapidly and efficiently prepare the skin cell suspension liquid in an operating room, the skin cell suspension liquid is directly used for treatment of skin defect diseases, complex laboratory personnel and equipment are not needed, the operation process is simple and rapid, and the risk of microbial contamination of bacteria and the like is greatly reduced.

Description

technical field [0001] The invention relates to a skin cell suspension preparation device and a preparation method thereof, belonging to the technical field of medical devices. Background technique [0002] The existing skin cell suspension preparation is carried out in the laboratory, and the preparation process is as follows: put the skin tissue to be digested into a centrifuge tube, add digestive enzyme solution, place it in a 37°C water bath or incubator, and shake continuously After the digestion is completed, filter, centrifuge the liquid, discard the supernatant, add Hanks solution or serum-free culture medium, centrifuge and rinse for 1-2 times, remove the supernatant, add culture medium to make a cell suspension, the operation process is more complicated and requires special The personnel and equipment just can be finished, and it is extremely inconvenient to use clinically. Contents of the invention [0003] In view of the above prominent problems, the present i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/38C12M1/33C12M1/12C12M1/00A61K35/36A61P17/02A61P17/00
CPCC12M21/18C12M21/08C12M23/02C12M39/00C12M41/12C12M33/14C12M45/02A61K35/36A61P17/02A61P17/00
Inventor 李洪生
Owner 李洪生
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