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Method for identifying mature rape honey

A technology for honey and rape, applied in the field of honey identification, can solve problems such as disrupting market order, inappropriate identification of users, and harming consumers' interests.

Active Publication Date: 2020-07-28
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, the cost is low, the output is high, and the identification of mature honey is cumbersome, and it is not suitable for users to identify
Therefore, the vast majority of honey on the market is not mature honey, but processed and concentrated immature honey, which seriously disrupts the market order and damages the interests of consumers.

Method used

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  • Method for identifying mature rape honey
  • Method for identifying mature rape honey
  • Method for identifying mature rape honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The present embodiment provides a kind of identification method of ripe rapeseed honey, comprises the steps:

[0030] S1. Collect natural capped mature rapeseed honey from the bee farm as the sample to be tested

[0031] S2, the sample to be tested is pretreated to obtain honey extract

[0032] 1) Weigh 5g of rapeseed honey to be tested, put the honey into a 50mL centrifuge tube, add 10mL of pure water, and ultrasonically dissolve it for 20min to obtain an aqueous solution of honey;

[0033] 2) Install the SPE column on the solid-phase extraction device, wash it with 5mL methanol and 5mL pure water respectively, and then add the honey solution, and the effective substances in the honey are adsorbed on the SPE column packing;

[0034] 3) The SPE column adsorbed with the effective substance of honey was first rinsed twice with 10 mL of pure water, then eluted with 8 mL of methanol, and the eluate was collected;

[0035] 4) Concentrate the eluent to a solid with a nitrog...

Embodiment 2

[0050] Collect uncapped immature rapeseed honey from the bee farm as the sample to be tested, and detect it by the method described in Implementation 1. As a result, the total ion pattern of the immature rapeseed honey extract Q-TOF analysis is as follows figure 1 As shown in A, the extracted chromatogram of immature rapeseed honey extract Q-TOF analysis characteristic ion 207.0663 is as follows image 3 shown.

[0051] From figure 2 and image 3 The comparison shows that the quasi-molecular ion peak with a mass number of 207.07±0.01 can be used as a characteristic peak to identify mature rapeseed honey, and only mature rapeseed honey contains this characteristic peak, while immature rapeseed honey does not have this characteristic peak. After repeated experiments, the results remained the same.

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Abstract

The invention provides a method for identifying mature rape honey. The method comprises the following steps that: to-be-detected honey is detected by adopting liquid chromatography-quadrupole rod-time-of-flight mass spectrometry; and excimer ion peaks with the mass number of 207.07 + / -0.01 are adopted as characteristic peaks, when it is judged that the to-be-detected honey is mature rape honey when the time-of-flight mass spectrometry contains the characteristic peaks. According to the method, it is determined that the excimer ion peaks with the mass number of 207.07 + / -0.01 can serve as the characteristic peaks for identifying the mature rape honey, only the mature rape honey contains the characteristic peaks, and non-mature rape honey does not contain the characteristic peaks. The methodfor identifying the mature rape honey is quick and accurate, has important significance for identifying the mature rape honey, and provides a guarantee for controlling the quality of the honey and maintaining the market order.

Description

technical field [0001] The invention belongs to the field of honey identification, in particular to a method for identifying mature rapeseed honey. Background technique [0002] Honey is a natural sweet substance that bees collect plant nectar or secretions, mix with their own secretions and fully brew. The smell is fragrant and strong, and the taste is pure and sweet. [0003] Honey is divided into mature honey and immature honey. Ripe honey refers to honey that has been fully brewed by bees. After the bees have brewed the honey, they will store it centrally in the hive and seal it with beeswax. To get honey at this time, the honey cover must be cut off with a honey cutter, which is very labor-intensive. Such honey will crystallize after a long period of time (coagulation of the whole honey), without any processing, and can be stored for a long time without deterioration. Non-mature honey has not been fully brewed, the brewing time is short, the water content in the ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N2030/062
Inventor 赵柳微王凯郭娜娜吴黎明薛晓锋彭文君
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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