Extraction method for insect protein

Abstract

The invention discloses an extraction method for insect protein. The extraction method comprises the following steps of: S1, carrying out primary treatment: selecting an integrally undamaged insect body, and cleaning, sterilizing and drying the insect body to obtain a primary treatment object; S2, carrying out secondary treatment: smashing and grinding the primary treatment object to obtain a smashing object, and filtering and screening the smashing object to obtain a secondary treatment object; S3, carrying out stirring and mixing: putting the obtained secondary treatment object in a stirrer,adding a proper quantity of distilled water, and then, carrying out heating and stirring; S4, carrying out degreasing: carrying out extraction and degreasing on liquid in the stirrer by a supercritical fluid carbon dioxide extraction method; and S5, carrying out hydrolysis: adding acetone into obtained degreased substances, carrying out centrifugation and layering by a centrifugal machine, obtaining total protein on a lower layer, adopting tris(hydroxymethyl) aminomethane to balance phenol enrichment, and then, carrying out second-time centrifugation. The extraction method for the insect protein is simple to operate, has few processes and can guarantee the quality and the activity of the protein.

Description

Technical field [0001] The invention belongs to the technical field of protein extraction, and specifically relates to an insect protein extraction method. Background technique [0002] The protein content of insects is high. The protein content of most insect dry bodies is as high as 40% or more. For example, dried wasps contain about 81% protein, bees 43%, cicadas 72%, grasshoppers 70%, crickets 65%, and rice locusts 60%. %, 52% of tussah pupa. Insect protein is rich in various amino acids required by the animal body, and the ratio of essential amino acids is similar to that established by the Food and Agriculture Organization of the United Nations (FAO), making it an ideal source of feed protein. In addition, insect protein is also rich in various amino acids needed by the human body, especially lysine, threonine, methionine, etc. that cannot be synthesized by itself, so people pay attention to it. People have been experimenting with extracting proteins from insects since th...

AI Technical Summary

Problems solved by technology

[0003] The more popular insect protein extraction is generally alkaline extraction, but under high alkali conditions, protein hydrolysis will be caused, the non-protein content in the extract will increase, and the separation effect will decrease.